Cooperative interactions at the SLP-76 complex are critical for actin polymerization

Mira Barda-Saad; Naoto Shirasu; Maor H Pauker; Nirit Hassan; Orly Perl; Andrea Balbo; Hiroshi Yamaguchi; Jon C D Houtman; Ettore Appella; Peter Schuck; Lawrence E Samelson

doi:10.1038/emboj.2010.133

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Cooperative interactions at the SLP-76 complex are critical for actin polymerization

Journal article

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Cooperative interactions at the SLP-76 complex are critical for actin polymerization

Mira Barda-Saad, Naoto Shirasu, Maor H Pauker, Nirit Hassan, Orly Perl, Andrea Balbo, Hiroshi Yamaguchi, Jon C D Houtman, Ettore Appella, Peter Schuck, …

The EMBO journal, Vol.29(14), pp.2315-2328

07/21/2010

DOI: 10.1038/emboj.2010.133

PMCID: PMC2910278

PMID: 20562827

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Abstract

T-cell antigen receptor (TCR) engagement induces formation of multi-protein signalling complexes essential for regulating T-cell functions. Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP-76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C-terminal SH3 domain of Nck and the VAV1 N-terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T-cell activation.

Oncogene Proteins - genetics

Proto-Oncogene Proteins c-vav - genetics

Receptors, Antigen, T-Cell - metabolism

Jurkat Cells

Lymphocyte Activation

Humans

Actins - metabolism

Oncogene Proteins - metabolism

Phosphoproteins - genetics

Phosphoproteins - metabolism

Recombinant Fusion Proteins - metabolism

src Homology Domains

Animals

T-Lymphocytes - cytology

T-Lymphocytes - metabolism

Adaptor Proteins, Signal Transducing - genetics

Protein Binding

Recombinant Fusion Proteins - genetics

Signal Transduction - physiology

Adaptor Proteins, Signal Transducing - metabolism

Proto-Oncogene Proteins c-vav - metabolism

Details

Title: Subtitle: Cooperative interactions at the SLP-76 complex are critical for actin polymerization
Creators: Mira Barda-Saad - Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. bardasm@mail.biu.ac.il
Naoto Shirasu
Maor H Pauker
Nirit Hassan
Orly Perl
Andrea Balbo
Hiroshi Yamaguchi
Jon C D Houtman
Ettore Appella
Peter Schuck
Lawrence E Samelson
Resource Type: Journal article
Publication Details: The EMBO journal, Vol.29(14), pp.2315-2328
DOI: 10.1038/emboj.2010.133
PMID: 20562827
PMCID: PMC2910278
ISSN: 0261-4189
eISSN: 1460-2075
Grant note: Intramural NIH HHS
Language: English
Date published: 07/21/2010
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984094556302771

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