Journal article
Coordination and modulation of locomotion pattern generators in Drosophila larvae: effects of altered biogenic amine levels by the tyramine beta hydroxlyase mutation
The Journal of neuroscience, Vol.26(5), pp.1486-1498
02/01/2006
DOI: 10.1523/JNEUROSCI.4749-05.2006
PMCID: PMC2673197
PMID: 16452672
Abstract
Forward locomotion of Drosophila melanogaster larvae is composed of rhythmic waves of contractions that are thought to be produced by segmentally organized central pattern generators. We present a systematic description of spike activity patterns during locomotive contraction waves in semi-intact wild-type and mutant larval preparations. We have shown previously that Tbetah(nM18) mutants, with altered levels of octopamine and tyramine, have a locomotion deficit. By recording en passant from the segmental nerves, we investigated the coordination of the neuronal activity driving contraction waves of the abdominal body-wall muscles. Rhythmic bursts of activity that occurred concurrently with locomotive waves were frequently observed in wild-type larvae but were rarely seen in Tbetah(nM18) mutants. These centrally generated patterned activities were eliminated in the distal stumps of both wild-type and Tbetah(nM18) larvae after severing the segmental nerve from the CNS. Patterned activities persisted in the proximal stumps deprived of sensory feedback from the periphery. Simultaneous recordings demonstrated a delay in the bursting activity between different segments, with greater delay for segments that were farther apart. In contrast, bilateral recordings within a single segment revealed a well synchronized activity pattern in nerves innervating each hemisegment in both wild-type and Tbetah(nM18) larvae. Significantly, rhythmic patterns of bursts and waves could be evoked in Tbetah(nM18) mutants by head or tail stimulation despite their highly irregular spontaneous activities. These observations suggest a role of the biogenic amines in the initiation and modulation of motor pattern generation. The technique presented here can be readily extended to examine the locomotion motor program of other mutants.
Details
- Title: Subtitle
- Coordination and modulation of locomotion pattern generators in Drosophila larvae: effects of altered biogenic amine levels by the tyramine beta hydroxlyase mutation
- Creators
- Lyle E Fox - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA. lyle-fox@uiowa.eduDavid R SollChun-Fang Wu
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.26(5), pp.1486-1498
- Publisher
- United States
- DOI
- 10.1523/JNEUROSCI.4749-05.2006
- PMID
- 16452672
- PMCID
- PMC2673197
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Grant note
- R01 NS026528 / NINDS NIH HHS HD18577 / NICHD NIH HHS R01 NS026528-15 / NINDS NIH HHS R01 NS026528-14 / NINDS NIH HHS NS26528 / NINDS NIH HHS P01 HD018577-210003 / NICHD NIH HHS P01 HD018577-200003 / NICHD NIH HHS P01 HD018577 / NICHD NIH HHS
- Language
- English
- Date published
- 02/01/2006
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070110102771
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