Journal article
Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells
Advanced NanoBiomed Research (Online), Vol.1(4), pp.2000062-n/a
02/22/2021
DOI: 10.1002/anbr.202000062
Abstract
Copper oxide (CuO) nanoparticles (NPs) are abundant in manufacturing processes, but they are an airway irritant. In vitro pulmonary toxicity of CuO NPs has been modeled using cell lines such as human bronchial epithelial cell line BEAS-2B. In 2D in vitro culture, BEAS-2B undergoes squamous differentiation due to the presence of serum. Differentiation is part of the repair process of lung cells in vivo that helps to preserve the epithelial lining of the respiratory tract. Herein, the effects of serum on the hydrodynamic diameter, cellular viability, cellular differentiation, and cellular uptake of 5 and 35 nm CuO NPs are investigated, and the mean cell area is used as the differentiation marker for BEAS-2B cells. The results demonstrate that the hydrodynamic diameter decreases with the addition of serum to the culture medium. Serum also increases the mean cell area, and only affects dose-dependent cytotoxicity of 35 nm CuO NPs, while simultaneously having no effect on intracellular Cu2+. This study presents evidence that both NP size and the presence of serum in culture media influence the relative viability of BEAS-2B cells following CuO NP exposure and highlights a critical need for carefully designed experiments and accurately reported conditions.
Details
- Title: Subtitle
- Copper Oxide Nanoparticle Diameter Mediates Serum‐Sensitive Toxicity in BEAS‐2B Cells
- Creators
- Angie S Morris - Department of Pharmaceutical Sciences College of Pharmacy University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Department of Chemistry College of Liberal Arts and Sciences University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USABrittany E Givens - Department of Pharmaceutical Sciences College of Pharmacy University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Department of Chemical and Biochemical Engineering College of Engineering University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Department of Chemical and Materials Engineering College of Engineering University of Kentucky Lexington KY 40506 USAAaron Silva - Department of Pharmaceutical Sciences College of Pharmacy University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Roy J. Carver Department of Biomedical Engineering College of Engineering University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USAAliasger K Salem - Department of Pharmaceutical Sciences College of Pharmacy University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Department of Chemistry College of Liberal Arts and Sciences University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Department of Chemical and Biochemical Engineering College of Engineering University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA, Roy J. Carver Department of Biomedical Engineering College of Engineering University of Iowa 115 S. Grand Avenue, S228 PHAR Iowa City IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Advanced NanoBiomed Research (Online), Vol.1(4), pp.2000062-n/a
- DOI
- 10.1002/anbr.202000062
- ISSN
- 2699-9307
- eISSN
- 2699-9307
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: P30CA086862
- Language
- English
- Date published
- 02/22/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984217440202771
Metrics
10 Record Views