Journal article
Copper intoxication inhibits aerobic nucleotide synthesis in Streptococcus pneumoniae
Metallomics, Vol.7(5), pp.786-794
05/01/2015
DOI: 10.1039/c5mt00011d
PMCID: PMC4431907
PMID: 25730343
Abstract
Copper is universally toxic in excess, a feature exploited by the human immune system to facilitate bacterial clearance. The mechanism of copper intoxication remains unknown for many bacterial species. Here, we demonstrate that copper toxicity in Streptococcus pneumoniae is independent from oxidative stress but, rather, is the result of copper inhibiting the aerobic dNTP biosynthetic pathway. Furthermore, we show that copper-intoxicated S. pneumoniae is rescued by manganese, which is an essential metal in the aerobic nucleotide synthesis pathway. These data provide insight into new targets to enhance copper-mediated toxicity during bacterial clearance.
Details
- Title: Subtitle
- Copper intoxication inhibits aerobic nucleotide synthesis in Streptococcus pneumoniae
- Creators
- Michael D. L. Johnson - St. Jude Children's Research HospitalThomas E. Kehl-Fie - University of Illinois Urbana-ChampaignJason W. Rosch - St. Jude Children's Research Hospital
- Resource Type
- Journal article
- Publication Details
- Metallomics, Vol.7(5), pp.786-794
- Publisher
- Royal Soc Chemistry
- DOI
- 10.1039/c5mt00011d
- PMID
- 25730343
- PMCID
- PMC4431907
- ISSN
- 1756-5901
- eISSN
- 1756-591X
- Number of pages
- 9
- Grant note
- American Lebanese Syrian Associated Charities (ALSAC) NIH K22AI104805-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AI110618 / NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Language
- English
- Date published
- 05/01/2015
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984618643102771
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