Journal article
Coronary microvascular response to endothelin is dependent on vessel diameter and route of administration
American journal of physiology. Heart and circulatory physiology, Vol.263(3), pp.H703-H709
09/01/1992
DOI: 10.1152/ajpheart.1992.263.3.H703
PMID: 1415594
Abstract
Endothelin is a 21-amino acid peptide originally isolated from vascular endothelial cells. In the present study, we examined the effect of topical and intracoronary administration of endothelin-1 on the coronary microcirculation and the effect of inhibition of cyclooxygenase on the microvascular response to intracoronary endothelin. In anesthetized dogs (n = 39), the coronary microcirculation was visualized using stroboscopic epi-illumination synchronized to the cardiac cycle. Topical application of endothelin [(5 x 10(-9) to 10(-8) M] constricted all arteries and arterioles with the degree of constriction inversely related to vessel size. Coronary veins and venules did not constrict to endothelin. Topical application of EDTA (10 mg/ml) reversed the constriction to endothelin in arterioles of all sizes. In contrast, intracoronary administration of endothelin (10(-8) to 10(-7) M) produced dilation of small arterioles (less than 130 microns) and no response of large arterioles (greater than 130 microns). The response of small arterioles to intracoronary endothelin was not altered by inhibition of cyclooxygenase with indomethacin (5 mg/kg); however, large arterioles constricted. Thus the coronary microvascular response to endothelin is dependent on the route of administration. Constriction of arteries and arterioles of all sizes to endothelin is dependent on extracellular calcium. Vasodilator prostaglandins may be released in response to intracoronary administration of endothelin predominantly in larger vessels. Thus the differential response to endothelin with topical and intracoronary administration may reflect a diffusional barrier of the endothelium or release of endothelium-derived relaxing factor and prostaglandins in response to endothelin.
Details
- Title: Subtitle
- Coronary microvascular response to endothelin is dependent on vessel diameter and route of administration
- Creators
- Kathryn G Lamping - Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City 52242Jennifer L Clothier - Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City 52242Charles L Eastham - Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City 52242Melvin L Marcus - Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.263(3), pp.H703-H709
- DOI
- 10.1152/ajpheart.1992.263.3.H703
- PMID
- 1415594
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 09/01/1992
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984094482502771
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