Coronavirus-induced demyelination occurs in the presence of virus-specific cytotoxic T cells

R F Castro; G D Evans; A Jaszewski; S Perlman

doi:10.1006/viro.1994.1237

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Coronavirus-induced demyelination occurs in the presence of virus-specific cytotoxic T cells

Journal article

Open access

Peer reviewed

Coronavirus-induced demyelination occurs in the presence of virus-specific cytotoxic T cells

R F Castro, G D Evans, A Jaszewski and S Perlman

Virology, Vol.200(2), pp.733-743

05/01/1994

DOI: 10.1006/viro.1994.1237

PMID: 8178457

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Abstract

C57BI/6, but not BALB/c, mice infected with mouse hepatitis virus strain JHM (MHV-JHM) develop a late onset, symptomatic demyelinating encephalomyelitis. In this report, we characterized anti-viral cytotoxic T cells in the central nervous system and spleen during the acute and chronic stages of the MHV infection. The data show that C57BI/6 mice display a cytotoxic T cell (CTL) response to the surface (S) glycoprotein and this response can be demonstrated in lymphocytes isolated from the brains and spinal cords of mice both acutely and persistently infected with MHV-JHM. Thus, the anti-S CTL activity present in the central nervous system of chronically infected animals is not sufficient to prevent the demyelinating process. BALB/c mice have been shown previously to mount a CTL response against the nucleocapsid (N) protein (Stohlman et al., 1992). Since C57BI/6 mice do not mount a response to the N protein, the role of the N-specific response in preventing the late onset disease was assessed using B10.A(18R) mice, recombinant in the H-2 locus. These mice contain the d alleles of the D and L loci and exhibit a CTL response against the N protein. However, unlike the BALB/c mice, these animals develop the late onset symptomatic disease. These results suggest that the N-specific response is partially protective against the development of the demyelinating disease, but that additional factors are also likely to be involved.

T-Lymphocyte Subsets - immunology

Acute Disease

Species Specificity

Mice, Inbred C57BL

Central Nervous System - pathology

T-Lymphocytes, Cytotoxic

Coronavirus Infections - immunology

Central Nervous System - immunology

CD8 Antigens - immunology

Murine hepatitis virus - immunology

Spike Glycoprotein, Coronavirus

Capsid - immunology

Animals

H-2 Antigens - genetics

Central Nervous System - cytology

Demyelinating Diseases - immunology

Encephalomyelitis - immunology

Viral Core Proteins - immunology

Viral Envelope Proteins - immunology

Mice

Mice, Inbred BALB C

Membrane Glycoproteins

Chronic Disease

Details

Title: Subtitle: Coronavirus-induced demyelination occurs in the presence of virus-specific cytotoxic T cells
Creators: R F Castro - Department of Microbiology, University of Iowa, Iowa City 52242
G D Evans
A Jaszewski
S Perlman
Resource Type: Journal article
Publication Details: Virology, Vol.200(2), pp.733-743
DOI: 10.1006/viro.1994.1237
PMID: 8178457
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: NS24401 / NINDS NIH HHS
Language: English
Date published: 05/01/1994
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777472402771

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