Correlation between Broth Microdilution and Disk Diffusion Results when Testing Ceftazidime-Avibactam against a Challenge Collection of Enterobacterales Isolates: Results from a Multilaboratory Study

Helio S Sader; Paul R Rhomberg; Sukantha Chandrasekaran; Marisol Trejo; Kelley A Fedler; Linda D Boyken; Daniel J Diekema

doi:10.1128/JCM.01757-19

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Correlation between Broth Microdilution and Disk Diffusion Results when Testing Ceftazidime-Avibactam against a Challenge Collection of Enterobacterales Isolates: Results from a Multilaboratory Study

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Correlation between Broth Microdilution and Disk Diffusion Results when Testing Ceftazidime-Avibactam against a Challenge Collection of Enterobacterales Isolates: Results from a Multilaboratory Study

Helio S Sader, Paul R Rhomberg, Sukantha Chandrasekaran, Marisol Trejo, Kelley A Fedler, Linda D Boyken and Daniel J Diekema

Journal of clinical microbiology, Vol.58(4), e01757-19

03/25/2020

DOI: 10.1128/JCM.01757-19

PMID: 31996445

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https://www.ncbi.nlm.nih.gov/pmc/articles/7098758View

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Abstract

We assessed the ceftazidime-avibactam disk diffusion breakpoints that provide the lowest discrepancy error rates by testing an isolate collection with ceftazidime-avibactam MIC values near the breakpoints. Isolates ( = 112) were susceptibility tested by broth microdilution and disk diffusion methods in 3 laboratories. Current disk diffusion breakpoints (≥21/≤20 mm for susceptible/resistant) provided the lowest error rates, but confirmatory MIC testing is indicated for isolates with inhibition zones of 20 to 22 mm.

disk diffusion

ceftazidime-avibactam

CRE

Enterobacteriaceae

KPC

Details

Title: Subtitle: Correlation between Broth Microdilution and Disk Diffusion Results when Testing Ceftazidime-Avibactam against a Challenge Collection of Enterobacterales Isolates: Results from a Multilaboratory Study
Creators: Helio S Sader - JMI Laboratories, North Liberty, Iowa, USA helio-sader@jmilabs.com
Paul R Rhomberg - JMI Laboratories, North Liberty, Iowa, USA
Sukantha Chandrasekaran - University of California, Los Angeles, Los Angeles, California, USA
Marisol Trejo - University of California, Los Angeles, Los Angeles, California, USA
Kelley A Fedler - JMI Laboratories, North Liberty, Iowa, USA
Linda D Boyken - University of Iowa, Iowa City, Iowa, USA
Daniel J Diekema - University of Iowa, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.58(4), e01757-19
DOI: 10.1128/JCM.01757-19
PMID: 31996445
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Grant note: DOI: 10.13039/100004319, name: Pfizer; DOI: 10.13039/100007819, name: Allergan
Language: English
Date published: 03/25/2020
Academic Unit: Infectious Diseases; Pathology; Internal Medicine
Record Identifier: 9984001235702771

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