Correlation of MTDH/AEG-1 and HOTAIR Expression with Metastasis and Response to Treatment in Sarcoma Patients

Mohammed M Milhem; Tina Knutson; Shujie Yang; Danlin Zhu; Xinjun Wang; Kimberly K Leslie; Xiangbing Meng

doi:10.4172/1948-5956.S5-004

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Correlation of MTDH/AEG-1 and HOTAIR Expression with Metastasis and Response to Treatment in Sarcoma Patients

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Correlation of MTDH/AEG-1 and HOTAIR Expression with Metastasis and Response to Treatment in Sarcoma Patients

Mohammed M Milhem, Tina Knutson, Shujie Yang, Danlin Zhu, Xinjun Wang, Kimberly K Leslie and Xiangbing Meng

Journal of cancer science & therapy, Vol.S5, 004

12/29/2011

DOI: 10.4172/1948-5956.S5-004

PMCID: PMC3612017

PMID: 23543869

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https://www.ncbi.nlm.nih.gov/pmc/articles/3612017View

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Abstract

Chemoresistance and metastasis are the main reasons for the failure of current treatments with sarcoma patients. Novel biomarkers are required to predict metastasis and response to treatment. The oncogene MTDH/AEG1 and the long noncoding RNA (lincRNA) HOTAIR are two novel factors involved in drug resistance and metastasis in various types of solid tumors. However, the correlation between MTDH/AEG-1 and HOTAIR expression with metastasis and drug resistance in sarcoma is unknown. Expression of MTDH protein or HOTAIR was detected by Western blotting or qRT-PCR, respectively, in primary and metastatic sarcoma patient tissue samples. High individual or co-expression of MTDH/AEG1 and HOTAIR was observed in three of four primary and six of eight metastatic sarcoma patient tumor samples. High level expression of both of MTDH/AEG1 and HOTAIR in the primary tumor correlated with a likelihood to metastasize. MTDH expression was lower in samples pre-treated with irradiation and/or chemotherapy as compared to those that had not been treated. HOTAIR expression seemed to correlate with the percent necrosis seen in different sarcoma samples. High levels of both MTDH/AEG-1 and HOTAIR in primary sarcoma are correlated with a high probability of metastasis. By contrast, reduced expression of both MTDH/AEG-1 and HOTAIR is correlated with a good response to treatment in terms of necrosis, suggesting that levels of MTDH and HOTAIR are potential biomarkers for treatment efficacy. Whether we can predict disease progression in sarcoma remains to be seen. Additional study is needed to better define the best clinical application of MTDH/AEG-1 and HOTAIR expression with metastasis and outcome.

Metastasis

HOTAIR

Sarcoma

MTDH/AEG-1

Details

Title: Subtitle: Correlation of MTDH/AEG-1 and HOTAIR Expression with Metastasis and Response to Treatment in Sarcoma Patients
Creators: Mohammed M Milhem - Department of Internal Medicine, University of Iowa, Iowa City, USA ; Holden Comprehensive Cancer Center, University of Iowa, Iowa City, USA
Tina Knutson
Shujie Yang
Danlin Zhu
Xinjun Wang
Kimberly K Leslie
Xiangbing Meng
Resource Type: Journal article
Publication Details: Journal of cancer science & therapy, Vol.S5, 004
DOI: 10.4172/1948-5956.S5-004
PMID: 23543869
PMCID: PMC3612017
NLM abbreviation: J Cancer Sci Ther
ISSN: 1948-5956
eISSN: 1948-5956
Publisher: United States
Grant note: R01 CA062483-28S1 / NCI NIH HHS R01 CA099908 / NCI NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 12/29/2011
Academic Unit: Pathology; Orthopedics and Rehabilitation; Obstetrics and Gynecology; Internal Medicine
Record Identifier: 9983930877702771

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