Journal article
Cortical localization of a calcium release channel in sea urchin eggs
The Journal of cell biology, Vol.116(5), pp.1111-1121
03/01/1992
DOI: 10.1083/jcb.116.5.1111
PMCID: PMC2289353
PMID: 1310992
Abstract
We have used an antibody against the ryanodine receptor/calcium release channel of skeletal muscle sarcoplasmic reticulum to localize a calcium release channel in sea urchin eggs. The calcium release channel is present in less than 20% of immature oocytes, where it does not demonstrate a specific cytoplasmic localization, while it is confined to the cortex of all mature eggs examined. This is in contrast to the cortical and subcortical localization of calsequestrin in mature and immature eggs. Immunolocalization of the calcium release channel reveals a cortical reticulum or honeycomb staining network that surrounds cortical granules and is associated with the plasma membrane. The network consists of some immunoreactive electron-dense material coating small vesicles and elongate cisternae of the endoplasmic reticulum. The fluorescent reticular staining pattern is lost when egg cortices are treated with agents known to affect sarcoplasmic reticulum calcium release and induce cortical granule exocytosis (ryanodine, calcium, A-23187, and caffeine). An approximately 380-kD protein of sea urchin egg cortices is identified by immunoblot analysis with the ryanodine receptor antibody. These results demonstrate: (a) the presence of a ryanodine-sensitive calcium release channel that is located within the sea urchin egg cortex; (b) an altered calcium release channel staining pattern as a result of treatments that initiate the cortical granule reaction; and (c) a spatial and functional dichotomy of the ER which may be important in serving different roles in the mobilization of calcium at fertilization.
Details
- Title: Subtitle
- Cortical localization of a calcium release channel in sea urchin eggs
- Creators
- Sandra M McPherson - Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242Peter S Mcpherson - Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242Lori Mathews - Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242Kevin P Campbell - Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242Frank J Longo - Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.116(5), pp.1111-1121
- DOI
- 10.1083/jcb.116.5.1111
- PMID
- 1310992
- PMCID
- PMC2289353
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 03/01/1992
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Iowa Neuroscience Institute
- Record Identifier
- 9984068280002771
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