Journal article
Coupling between the N- and C-Terminal Domains Influences Transducin-α Intrinsic GDP/GTP Exchange
Biochemistry (Easton), Vol.39(14), pp.3937-3942
04/11/2000
DOI: 10.1021/bi992156l
PMID: 10747781
Abstract
The N-terminal regions of the heterotrimeric G-protein α-subunits represent one of the major Gβγ contact sites and have been implicated in an interaction with G-protein-coupled receptors. To probe the role of the N-terminal domain of transducin-α in G-protein function, a chimeric Gtiα subunit with the 31 N-terminal Gtα residues replaced by the corresponding 42 residues of Gsα (Ns-Gtiα) has been examined for the interaction with light-activated rhodopsin (R*). Gtiα displayed a somewhat higher R*-stimulated rate of GTPγS binding relative to Ns-Gtiα, suggesting modest involvement of the Gtα N-terminal sequence in recognition of the receptor. However, the intrinsic rate of nucleotide exchange in Ns-Gtiα was significantly faster (k app = 0.014 min-1) than that in Gtiα (k app = 0.0013 min-1) as judged by the GTPγS binding rates. Substitution of 42 N-terminal residues of Gsα by the Gtα residues in a reciprocal chimera, Nt-Gsα, had an opposite effectnotable reduction in the intrinsic GTPγS-binding rate (k app = 0.0075 min-1) in comparison with Gsα (k app = 0.028 min-1). Residue Val30 (His41 in Gsα) within the N-terminal region of Gtα interacts with the C-terminal residue, Ile339. To test the hypothesis that observed changes in the intrinsic nucleotide exchange rate in chimeric Gα subunits might be attributed to this interaction, GtiαVal30His, GtiαIle339Ala, and Ns-GtiαHis41Val mutants have been made and analyzed for basal GTPγS binding. GtiαVal30His and GtiαIle339Ala had increased GTPγS binding rates (k app = 0.010 and 0.009 min-1, respectively), whereas Ns-GtiαHis41Val had a decreased GTPγS binding rate (k app = 0.0011 min-1) relative to their parent proteins. These results suggest that the coupling between the N-terminal and C-terminal domains of Gtα is important for maintaining a low nucleotide exchange rate in unstimulated transducin.
Details
- Title: Subtitle
- Coupling between the N- and C-Terminal Domains Influences Transducin-α Intrinsic GDP/GTP Exchange
- Creators
- Khakim G MuradovNikolai O Artemyev
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.39(14), pp.3937-3942
- Publisher
- American Chemical Society
- DOI
- 10.1021/bi992156l
- PMID
- 10747781
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Language
- English
- Date published
- 04/11/2000
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025309602771
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