Journal article
Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95
Biochemical and biophysical research communications, Vol.425(1), pp.13-18
08/17/2012
DOI: 10.1016/j.bbrc.2012.07.033
PMCID: PMC3423544
PMID: 22809504
Abstract
► CAR indirectly interacts with ASIC3 via a PDZ-based interaction with PSD-95. ► CAR mediates junctional localization of ASIC3 and PSD-95. ► CAR rescues PSD-95-mediated inhibition of ASIC3 currents.
We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell–cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H+-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.
Details
- Title: Subtitle
- Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95
- Creators
- Katherine J.D.A. Excoffon - Wright State UniversityAbimbola O. Kolawole - Wright State UniversityNobuyoshi Kusama - Roy J. and Lucille A. Carver College of MedicineNicholas D. Gansemer - University of IowaPriyanka Sharma - Wright State UniversityAlesia M. Hruska-Hageman - Mount Mercy UniversityElena Petroff - Montclair State UniversityChristopher J. Benson - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.425(1), pp.13-18
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2012.07.033
- PMID
- 22809504
- PMCID
- PMC3423544
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 08/17/2012
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984303854502771
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