Journal article
CpG DNA increases primary malignant B cell expression of costimulatory molecules and target antigens
Journal of leukocyte biology, Vol.69(1), pp.81-88
01/2001
DOI: 10.1189/jlb.69.1.81
PMID: 11200072
Abstract
Multiple factors, including expression of costimulatory molecules, antigen‐presenting molecules, and target antigens, likely impact the efficacy of antibody therapy and other approaches to the immunotherapy of B cell malignancy. Unmethylated CpG‐dinucleotides in select base contexts (“CpG motifs”) that resemble sequences found in bacterial DNA are potent immunostimulatory agents capable of inducing a complex immune response, including a strong B cell stimulus. We examined the effect of a potent human CpG oligonucleotide (CpG ODN 2006) on different types of primary human malignant B cells and reactive follicular hyperplasia. CpG oligodeoxynucleotide (CpG ODN), but not control (non‐CpG ODN), increased the expression of costimulatory molecules (CD40, CD80, CD86, CD54) on malignant B cells without altering the phenotype of B cells obtained from reactive follicular hyperplasia. CpG ODN also enhanced expression of class I and class II MHC in most samples. CD20 expression was increased in response to CpG ODN, most notably in B‐CLL and marginal zone lymphoma. An inverse correlation was found between baseline expression of CD20 and CD40 and their expression after exposure to CpG ODN, thus the most significant increase in expression of these molecules was found in those samples that had the lowest baseline levels. In conclusion, CpG ODN can lead to increasing expression of molecules involved in costimulation, antigen presentation, and as targets for antibody‐based therapy and deserve further evaluation as potential immunotherapeutic agents for B cell malignancy.
Details
- Title: Subtitle
- CpG DNA increases primary malignant B cell expression of costimulatory molecules and target antigens
- Creators
- Bernd JahrsdörferGunther HartmannEmil RacilaWallen JacksonLars MühlenhoffGerold MeinhardtStefan EndresBrian K LinkArthur M KriegGeorge J Weiner
- Resource Type
- Journal article
- Publication Details
- Journal of leukocyte biology, Vol.69(1), pp.81-88
- DOI
- 10.1189/jlb.69.1.81
- PMID
- 11200072
- ISSN
- 0741-5400
- eISSN
- 1938-3673
- Number of pages
- 8
- Grant note
- Munich's Ludwig Maximilians University National Institutes of Health (R01 CA77764) University of Iowa Cancer Center CpG ImmunoPharmaceuticals GmbH, Hilden, Germany University of Munich Coley Pharmaceutical Group Inc. (BMBF 03‐12235‐6) German Israeli Foundation (I‐021‐203.05/96)
- Language
- English
- Date published
- 01/2001
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Infectious Disease (Pediatrics); Internal Medicine
- Record Identifier
- 9984094777802771
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