CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge

Arthur M Krieg; Laurie Love-Homan; Ae-Kyung Yi; John T Harty

doi:10.4049/jimmunol.161.5.2428

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CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge

Journal article

Peer reviewed

CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge

Arthur M Krieg, Laurie Love-Homan, Ae-Kyung Yi and John T Harty

The Journal of immunology (1950), Vol.161(5), pp.2428-2434

09/01/1998

DOI: 10.4049/jimmunol.161.5.2428

PMID: 9725240

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Abstract

Vertebrates have evolved innate immune defense mechanisms that recognize and respond to structural patterns that are specific to microbial molecules. One such pattern recognition system is based on unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs); these motifs are common in bacterial DNA but are under-represented ("CpG suppression") and methylated in vertebrate DNA. Mice that are injected with bacterial DNA or synthetic oligodeoxynucleotides (ODNs) containing CpG motifs respond with a rapid production of IL-12 and IFN-gamma. The serum levels of IL-12 were increased for at least 8 days after a single injection of CpG ODNs, but IFN-gamma levels returned to baseline within 24 h. This Th1-like cytokine response to CpG motifs induces a state of resistance to infection by Listeria monocytogenes in susceptible specific pathogen-free BALB/c mice. Resistance developed within 48 h of pretreatment with CpG ODNs, persisted for at least 2 wk, and was dependent upon IFN-gamma secretion. These data support the hypothesis that CpG DNA motifs are a "danger signal" that activates protective innate immune defenses and may have therapeutic potential.

Thionucleotides - immunology

Oligodeoxyribonucleotides - administration & dosage

CpG Islands - immunology

Oligodeoxyribonucleotides - immunology

Listeriosis - genetics

Injections, Intraperitoneal

Interferon-gamma - physiology

DNA, Bacterial - immunology

Specific Pathogen-Free Organisms - immunology

Immunity, Innate

Mice, Knockout

Interleukin-12 - blood

Thionucleotides - administration & dosage

Animals

Listeriosis - immunology

Female

Listeriosis - microbiology

DNA, Bacterial - administration & dosage

Mice

Mice, Inbred BALB C

Interferon-gamma - genetics

Kinetics

Interferon-gamma - deficiency

Interleukin-12 - biosynthesis

Details

Title: Subtitle: CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge
Creators: Arthur M Krieg - Interdisciplinary Graduate Program in Immunology, Department of Internal Medicine, University of Iowa, Iowa City 52242, USA. arthur-krieg@uiowa.edu
Laurie Love-Homan
Ae-Kyung Yi
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.161(5), pp.2428-2434
Publisher: United States
DOI: 10.4049/jimmunol.161.5.2428
PMID: 9725240
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: R29-AR42556-01 / NIAMS NIH HHS AI36864 / NIAID NIH HHS DK25295 / NIDDK NIH HHS
Language: English
Date published: 09/01/1998
Academic Unit: Pathology
Record Identifier: 9984047759802771

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