Journal article
CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen
The Journal of immunology (1950), Vol.160(2), pp.870-876
01/15/1998
DOI: 10.4049/jimmunol.160.2.870
PMID: 9551923
Abstract
Unmethylated CpG dinucleotides in bacterial DNA or synthetic oligodeoxynucleotides (ODN) cause B cell proliferation and Ig secretion, monocyte cytokine secretion, and activation of NK cell lytic activity and IFN-gamma secretion in vivo and in vitro. The potent immune activation by CpG ODN suggests possible utility for enhancing immune responses to vaccines. Mice immunized with recombinant hepatitis B virus surface Ag and a CpG ODN as an immune enhancer have titers of Abs against HBsAg (anti-HBs) that are five times higher than those of mice immunized with HBsAg and the standard adjuvant, aluminum hydroxide (alum). Ab titers in mice immunized with HBsAg and both CpG ODN plus alum were 35 times higher than the titers in mice immunized with alum alone, indicating a strong synergistic interaction between the CpG ODN and alum. ODN without CpG motifs had little or no immune-enhancing activity at the doses used herein. Alum induces a Th2 humoral response (mostly IgG1) and no CTL. In contrast, CpG ODN gives a strong Thl response with predominantly IgG2a Abs and CTL, even when mixed with alum. In vitro studies to determine possible mechanisms of CpG immune-enhancing effects show that CpG ODN induce expression of costimulatory molecules on Ag-presenting cells and drive B cell isotype switching in the appropriate cytokine milieu. These studies demonstrate that CpG ODN are promising new immune enhancers for vaccination applications.
Details
- Title: Subtitle
- CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen
- Creators
- Heather L Davis - Loeb Research Institute, Ottawa Civic Hospital, ON, Canada. hdavis@civich.ottawa.on.caRisini WeeratnaThomas J. WaldschmidtLorraine TygrettJoachim SchorrArthur M Krieg
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.160(2), pp.870-876
- DOI
- 10.4049/jimmunol.160.2.870
- PMID
- 9551923
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- R29AR42556 / NIAMS NIH HHS R01 AI31265 / NIAID NIH HHS DK25295 / NIDDK NIH HHS
- Language
- English
- Date published
- 01/15/1998
- Academic Unit
- Pathology
- Record Identifier
- 9984047606002771
Metrics
35 Record Views