Journal article
CpG island arrays : An application toward deciphering epigenetic signatures of breast cancer
Clinical cancer research, Vol.6(4), pp.1432-1438
2000
PMID: 10778974
Abstract
CpG island hypermethylation is a frequent epigenetic event in cancer. We have recently developed an array-based method, called differential methylation hybridization (DMH), allowing for a genome-wide screening of CpG island hypermethylation in breast cancer cell lines (T. H-M. Huang et al., Hum. Mol. Genet., 8: 459-470, 1999). In the present study, DMH was applied to screen 28 paired primary breast tumor and normal samples and to determine whether patterns of specific epigenetic alterations correlate with pathological parameters in the patients analyzed. Amplicons, representing a pool of methylated CpG DNA derived from these samples, were used as hybridization probes in an array panel containing 1104 CpG island tags. Close to 9% of these tags exhibited extensive hypermethylation in the majority of breast tumors relative to their normal controls, whereas others had little or no detectable changes. Pattern analysis in a subset of CpG island tags revealed that CpG island hypermethylation is associated with histological grades of breast tumors. Poorly differentiated tumors appeared to exhibit more hypermethylated CpG islands than their moderately or well-differentiated counterparts (P = 0.041). This early finding lays the groundwork for a population-based DMH study and demonstrates the need to develop a database for examining large-scale methylation data and for associating specific epigenetic signatures with clinical parameters in breast cancer.
Details
- Title: Subtitle
- CpG island arrays : An application toward deciphering epigenetic signatures of breast cancer
- Creators
- Pearlly S Yan - Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United StatesMartin R Perry - Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United StatesDouglas E Laux - Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United StatesAdam L Asare - Department of Health Management and Informatics, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United StatesCharles W Caldwell - Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United StatesTim Hui-Ming Huang - Department of Pathology and Anatomical Sciences, Ellis Fischel Cancer Center, University of Missouri School of Medicine, Columbia, Missouri 65203, United States
- Resource Type
- Journal article
- Publication Details
- Clinical cancer research, Vol.6(4), pp.1432-1438
- Publisher
- American Association for Cancer Research
- PMID
- 10778974
- ISSN
- 1078-0432
- eISSN
- 1557-3265
- Language
- English
- Date published
- 2000
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094772502771
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