Journal article
CpG motifs in bacterial DNA trigger direct B-cell activation
Nature (London), Vol.374(6522), pp.546-549
04/1995
DOI: 10.1038/374546a0
PMID: 7700380
Abstract
Unmethylated CpG dinucleotides are more frequent in the genomes of bacteria and viruses than of vertebrates. We report here that bacterial DNA and synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides induce murine B cells to proliferate and secrete immunoglobulin in vitro and in vivo. This activation is enhanced by simultaneous signals delivered through the antigen receptor. Optimal B-cell activation requires a DNA motif in which an unmethylated CpG dinucleotide is flanked by two 5' purines and two 3' pyrimidines. Oligodeoxynucleotides containing this CpG motif induce more than 95% of all spleen B cells to enter the cell cycle. These data suggest a possible evolutionary link between immune defence based on the recognition of microbial DNA and the phenomenon of 'CpG suppression' in vertebrates. The potent immune activation by CpG oligonucleotides has implications for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.
Details
- Title: Subtitle
- CpG motifs in bacterial DNA trigger direct B-cell activation
- Creators
- Arthur M KriegAe-Kyung YiSara MatsonThomas J WaldschmidtGail A BishopRebecca TeasdaleGary A KoretzkyDennis M Klinman
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.374(6522), pp.546-549
- DOI
- 10.1038/374546a0
- PMID
- 7700380
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 04/1995
- Academic Unit
- Microbiology and Immunology; President; Pathology
- Record Identifier
- 9984001209802771
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