Journal article
CpG-oligodeoxynucleotide supports growth of IL-6-dependent 7TD1 murine hybridoma cells
Life sciences (1973), Vol.62(1), pp.23-27
1998
DOI: 10.1016/S0024-3205(97)01034-5
PMID: 9444964
Abstract
The mouse-mouse hybridoma plasma cell line 7TD1 requires exogenous IL-6 for growth, and has been used to characterize and assay IL-6. Oligodeoxynucleotides containing CpG (CpG-ODN) and bacterial DNA are immunostimulatory, preventing B-cell apoptosis and inducing cytokine synthesis. We report that the phosphorothioate CpG-ODN 5'-ATAATCGACGTTCAAGCAAG-3' (half maximal effect at 0.3 microg/ml) supports the growth of 7TD1 cells in the absence of added IL-6. A non-CpG control ODN was without effect. No IL-6 production by 7TD1 cells incubated with CpG-ODN was detected by sensitive immunoassay. CpG-ODN-supported growth was not influenced by IL-6-neutralizing antibody, but was completely abolished by chloroquine and quinacrine, agents which inhibit CpG-ODN responses in other cells. Our results show that CpG-ODN can substitute for IL-6 in this cell line. This cell line may be useful for assaying CpG-ODN activity and for screening congeners of chloroquine and quinacrine for their ability to inhibit CpG effects.
Details
- Title: Subtitle
- CpG-oligodeoxynucleotide supports growth of IL-6-dependent 7TD1 murine hybridoma cells
- Creators
- Lori Manzel - Department of Medicine, VA Medical Center, and University of Iowa, Iowa City 52242, USADonald E Macfarlane
- Resource Type
- Journal article
- Publication Details
- Life sciences (1973), Vol.62(1), pp.23-27
- DOI
- 10.1016/S0024-3205(97)01034-5
- PMID
- 9444964
- NLM abbreviation
- Life Sci
- ISSN
- 0024-3205
- eISSN
- 1879-0631
- Language
- English
- Date published
- 1998
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094773002771
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