Journal article
CpG oligodeoxynucleotides enhance FcγRI-mediated cross presentation by dendritic cells
International immunology, Vol.16(8), pp.1091-1098
08/2004
DOI: 10.1093/intimm/dxh110
PMID: 15192052
Abstract
Dendritic cells (DC) can trigger naive CD8+ T cell responses by their capacity to cross-present exogenous antigens via the major histocompatibility complex class I pathway. The myeloid class I IgG receptor, FcγRI (CD64), is expressed on DC, and in vivo targeting of antigens to FcγRI induces strong humoral and cellular immune responses. We studied the capacity of human FcγRI (hFcγRI) to facilitate DC-mediated cross presentation and T cell activation, and assessed the effect of CpG oligodeoxynucleotides on this process. We generated hFcγRI expressing immature DC from hFcγRI transgenic and immature DC from non-transgenic mice. Antigens were targeted to Fcγ receptors as ovalbumin immune complexes, or selectively to hFcγRI via ovalbumin–CD64 mAb fusion proteins. Co-incubation of immature DC with CpG ODN led to markedly increased MHC class I presentation of FcγR-targeted antigens. When OVA was selectively targeted to hFcγRI, few differences were observed between Tg and NTg DC. However, upon co-incubation with CpG ODN, hFcγRI-triggered cross presentation was enhanced. These results document the capacity of hFcγRI on DC to trigger cross presentation via MHC class I upon co-culture with CpG ODN.
Details
- Title: Subtitle
- CpG oligodeoxynucleotides enhance FcγRI-mediated cross presentation by dendritic cells
- Creators
- Lisette Bevaart - Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Lundlaan 6, Room KC02-085.2, 3584 EA, Utrecht, The NetherlandsHeidi H Van Ojik - Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Lundlaan 6, Room KC02-085.2, 3584 EA, Utrecht, The NetherlandsAmanda W Sun - Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USATimothy H Sulahian - Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756, USAJeanette H. W Leusen - Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Lundlaan 6, Room KC02-085.2, 3584 EA, Utrecht, The NetherlandsGeorge J Weiner - The Holden Comprehensive Cancer Center and Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAJan G. J van de Winkel - Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Lundlaan 6, Room KC02-085.2, 3584 EA, Utrecht, The NetherlandsMartine J Van Vugt - Immunotherapy Laboratory, Department of Immunology, University Medical Center Utrecht, Lundlaan 6, Room KC02-085.2, 3584 EA, Utrecht, The Netherlands
- Resource Type
- Journal article
- Publication Details
- International immunology, Vol.16(8), pp.1091-1098
- Publisher
- Oxford University Press
- DOI
- 10.1093/intimm/dxh110
- PMID
- 15192052
- ISSN
- 0953-8178
- eISSN
- 1460-2377
- Language
- English
- Date published
- 08/2004
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Pharmaceutical Sciences and Experimental Therapeutics; Internal Medicine
- Record Identifier
- 9984094349402771
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