Journal article
Creation of EGD-Derived Gastric Cancer Organoids to Predict Treatment Responses
Cancers, Vol.15(11), 3036
06/02/2023
DOI: 10.3390/cancers15113036
PMCID: PMC10252567
PMID: 37296998
Abstract
Gastric adenocarcinoma (GAd) is the third leading cause of cancer-related deaths worldwide. Most patients require perioperative chemotherapy, yet methods to accurately predict responses to therapy are lacking. Thus, patients may be unnecessarily exposed to considerable toxicities. Here, we present a novel methodology using patient-derived organoids (PDOs) that rapidly and accurately predicts the chemotherapy efficacy for GAd patients. Methods: Endoscopic GAd biopsies were obtained from 19 patients, shipped overnight, and PDOs were developed within 24 h. Drug sensitivity testing was performed on PDO single-cells with current standard-of-care systemic GAd regimens and cell viability was measured. Whole exome sequencing was used to confirm the consistency of tumor-related gene mutations and copy number alterations between primary tumors, PDOs, and PDO single-cells. Results: Overall, 15 of 19 biopsies (79%) were appropriate for PDO creation and single-cell expansion within 24 h of specimen collection and overnight shipment. With our PDO single-cell technique, PDOs (53%) were successfully developed. Subsequently, two PDO lines were subjected to drug sensitivity testing within 12 days from initial biopsy procurement. Drug sensitivity assays revealed unique treatment response profiles for combination drug regimens in both of the two unique PDOs, which corresponded with the clinical response. Conclusions: The successful creation of PDOs within 24 h of endoscopic biopsy and rapid drug testing within 2 weeks demonstrate the feasibility of our novel approach for future applications in clinical decision making. This proof of concept sets the foundation for future clinical trials using PDOs to predict clinical responses to GAd therapies.
Details
- Title: Subtitle
- Creation of EGD-Derived Gastric Cancer Organoids to Predict Treatment Responses
- Creators
- Hannah G. McDonald - University of KentuckyMegan M. Harper - University of KentuckyKristen Hill - University of KentuckyAnqi Gao - University of KentuckyAngelica L. Solomon - University of KentuckyCharles J. Bailey - University of KentuckyMiranda Lin - University of KentuckyMautin Barry-Hundeyin - University of KentuckyMichael J. Cavnar - University of KentuckySamuel H. Mardini - University of KentuckyPrakash J. Pandalai - University of KentuckyReema A. Patel - University of KentuckyJill M. Kolesar - University of KentuckyJustin A. Rueckert - University of KentuckyLawrence Hookey - Queen's UniversityMark Ropeleski - Queen's UniversityShaila J. Merchant - Queen's UniversityJoseph Kim - University of KentuckyMei Gao - University of Kentucky
- Resource Type
- Journal article
- Publication Details
- Cancers, Vol.15(11), 3036
- Publisher
- Mdpi
- DOI
- 10.3390/cancers15113036
- PMID
- 37296998
- PMCID
- PMC10252567
- ISSN
- 2072-6694
- eISSN
- 2072-6694
- Number of pages
- 11
- Grant note
- P30CA177558; T32CA160003 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA John Wayne Cancer Institute Research Foundation pilot award
- Language
- English
- Date published
- 06/02/2023
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984695792202771
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