Journal article
Cross-clade T lymphocyte-mediated immunity to HIV type 1: implications for vaccine design and immunodetection assays
AIDS research and human retroviruses, Vol.18(14), pp.1067-1079
09/20/2002
DOI: 10.1089/08892220260235425
PMID: 12396458
Abstract
Identifying immunodominant regions of HIV-1 that are recognized by CD8(+) T lymphocytes in infected individuals may be important for the design and evaluation of candidate HIV-1 vaccines, particularly for developing countries. In this study, cryopreserved peripheral blood mononuclear cells (PBMCs) from 15 chronically HIV-1-infected U.S. volunteers were screened for HIV-1 Gag-specific T lymphocyte interferon gamma production in an enzyme-linked immunospot (ELISpot) assay matrix format, using overlapping HIV-1 subtype A, B, and C Gag peptide pools. In the initial matrix screen, responses to HIV-1 subtype B Gag were detected in 11 of 15 (73%) of seropositive individuals and in none of 4 HIV-1-seronegative controls. There were differences in both the breadth and magnitudes of the responses observed in the matrix assay. Responses varied in breadth, ranging from broad responses (more than four peptides) of moderate magnitude (<100 spot-forming cells [SFCs]/10(5) PBMCs) to narrowly focused (two or fewer peptides), more potent responses (>150 SFC/10(5) PBMCs). Responses to A, B, and C clade peptides of HIV-1 Gag revealed that all responders to subtype B peptides were also found to recognize corresponding peptides from at least one of the other clades. The ability to recognize cross-clade peptides with one or two amino acid substitutions relative to the B clade peptide was both peptide and patient dependent. Overall, our results show that the ELISpot matrix algorithm described here may be an efficient approach for characterizing cross-clade CD8(+) T cell responses in either seropositive individuals or in seronegative HIV-1 vaccine recipients.
Details
- Title: Subtitle
- Cross-clade T lymphocyte-mediated immunity to HIV type 1: implications for vaccine design and immunodetection assays
- Creators
- Sheila M Keating - Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USARobert C BollingerThomas C QuinnJ Brooks JacksonLucy M Carruth
- Resource Type
- Journal article
- Publication Details
- AIDS research and human retroviruses, Vol.18(14), pp.1067-1079
- Publisher
- United States
- DOI
- 10.1089/08892220260235425
- PMID
- 12396458
- ISSN
- 0889-2229
- eISSN
- 1931-8405
- Grant note
- U01 A146745-01 / PHS HHS
- Language
- English
- Date published
- 09/20/2002
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984046812702771
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