Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies

Gregory A Bohach; Carolyn J Hovde; Jeffrey P Handley; Patrick M Schlievert

doi:10.1128/iai.56.2.400-404.1988

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Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies

Journal article

Open access

Peer reviewed

Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies

Gregory A Bohach, Carolyn J Hovde, Jeffrey P Handley and Patrick M Schlievert

Infection and immunity, Vol.56(2), pp.400-404

02/1988

DOI: 10.1128/iai.56.2.400-404.1988

PMCID: PMC259295

PMID: 2448242

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https://doi.org/10.1128/iai.56.2.400-404.1988View

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Abstract

We evaluated cross-reactivity of antibodies against staphylococcal and streptococcal pyrogenic toxins. Monoclonal antibodies against staphylococcal enterotoxin (ET) C1 and streptococcal pyrogenic exotoxin (SPE) A were tested for reactivity with homologous and heterologous pyrogenic toxins in vitro. Ten immunoglobulin G1 anti-ET C1 monoclonal antibodies showed little or no cross-reactivity in an enzyme-linked immunosorbent assay, but many of these could neutralize the mitogenic effect of ET B, SPE A, or both. Two immunoglobulin M anti-ET C1 monoclonal antibodies and eight immunoglobulin M anti-SPE A monoclonal antibodies showed extensive cross-reactivity in the enzyme-linked immunosorbent assay and the mitogenicity neutralization assay. No cross-reactivity was observed with SPE C or toxic shock syndrome toxin 1. Rabbits immunized against ET B, ET C1, or SPE A were resistant to challenge with the immunizing toxin. In addition, reciprocal immunity was stimulated by the two ETs, and immunity to SPE A provided protection against ET B but not ET C1. These results show that staphylococcal and streptococcal pyrogenic toxins which share sequence homology have common antigenic determinants which may not be detected in Ouchterlony immunodiffusion assays.

Rabbits

Staphylococcus - immunology

Animals

Lymphocyte Activation - drug effects

Bacterial Toxins - immunology

Pyrogens - immunology

Neutralization Tests

Staphylococcus aureus - immunology

Mice

Epitopes

Antibodies, Monoclonal - immunology

Details

Title: Subtitle: Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies
Creators: Gregory A Bohach - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455
Carolyn J Hovde
Jeffrey P Handley
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.56(2), pp.400-404
Publisher: United States
DOI: 10.1128/iai.56.2.400-404.1988
PMID: 2448242
PMCID: PMC259295
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: HL36611 / NHLBI NIH HHS
Language: English
Date published: 02/1988
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002393102771

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