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Cross-organ metabolite production and consumption in healthy and atherogenic conditions
Journal article   Peer reviewed

Cross-organ metabolite production and consumption in healthy and atherogenic conditions

Hosung Bae, Sunhee Jung, Johnny Le, Ian Tamburini, Joohwan Kim, Eric Wang, Won-Suk Song, Wonsuk Choi, Ki-Hong Jang, Taekyung Kang, …
Cell, Vol.188(16), pp.4441-4455.e16
08/07/2025
DOI: 10.1016/j.cell.2025.05.001
PMID: 40436017

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Abstract

Mammalian organs continuously produce and consume circulating metabolites for organismal health and survival. However, the landscape of this fundamental process and its perturbation by diet and disease is unknown. Using arteriovenous metabolomics, tissue transcriptomics, and hormone arrays in multiple pathophysiological conditions in pigs, we generated an atlas of 10 cross-organ metabolite production and consumption during fasting/feeding, Western diet, and cardiovascular disease progression induced by low-density lipoprotein receptor (LDLR) deficiency. We discovered numerous instances of feeding-dependent and -independent metabolite production and consumption by organs and proposed mechanisms by which these are disrupted by Western diet via altered metabolite concentration gradients and hormones. Both Western diet and LDLR deficiency trigger the release of bile acids (BAs) by extra-hepatic organs, likely contributing to abnormally elevated circulating BA levels and consequent vascular inflammation and atherosclerosis development. These resources reveal intricate inter-organ metabolic crosstalk across pathophysiological conditions, offering biochemical insights into diet effects and cardiometabolic diseases. [Display omitted] •An atlas of organ-specific metabolite production and consumption in pigs•Western diet compromises organ-specific metabolite production and consumption•Metabolite gradients and hormones may regulate metabolite production and consumption•Western diet and hypercholesterolemia induce bile acid release from extra-hepatic organs An atlas mapping organ-specific metabolite production and consumption across various pathophysiological states in pigs shows impaired metabolic activity across organs by a Western diet or hypercholesterolemia, including abnormal bile acid release from extra-hepatic tissues.
arteriovenous metabolomics bile acid metabolism cardiovascular disease circulating metabolite flux inter-organ exchange isotope tracing low-density lipoprotein receptor metabolomics western diet

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