Cross species genomic analysis identifies a mouse model as undifferentiated pleomorphic sarcoma/malignant fibrous histiocytoma

Jeffrey K Mito; Richard F Riedel; Leslie Dodd; Guy Lahat; Alexander J Lazar; Rebecca D Dodd; Lars Stangenberg; William C Eward; Francis J Hornicek; Sam S Yoon; Brian E Brigman; Tyler Jacks; Dina Lev; Sayan Mukherjee; David G Kirsch

doi:10.1371/journal.pone.0008075

Back

Cross species genomic analysis identifies a mouse model as undifferentiated pleomorphic sarcoma/malignant fibrous histiocytoma

Journal article

Open access

Peer reviewed

Cross species genomic analysis identifies a mouse model as undifferentiated pleomorphic sarcoma/malignant fibrous histiocytoma

Jeffrey K Mito, Richard F Riedel, Leslie Dodd, Guy Lahat, Alexander J Lazar, Rebecca D Dodd, Lars Stangenberg, William C Eward, Francis J Hornicek, Sam S Yoon, …

PloS one, Vol.4(11), pp.e8075-e8075

11/30/2009

DOI: 10.1371/journal.pone.0008075

PMCID: PMC2779485

PMID: 19956606

Files and links (1)

url

https://doi.org/10.1371/journal.pone.0008075View

Published (Version of record) Open Access

Abstract

Undifferentiated pleomorphic sarcoma/Malignant Fibrous Histiocytoma (MFH) is one of the most common subtypes of human soft tissue sarcoma. Using cross species genomic analysis, we define a geneset from the LSL-Kras(G12D); Trp53(Flox/Flox) mouse model of soft tissue sarcoma that is highly enriched in human MFH. With this mouse geneset as a filter, we identify expression of the RAS target FOXM1 in human MFH. Expression of Foxm1 is elevated in mouse sarcomas that metastasize to the lung and tissue microarray analysis of human MFH correlates overexpression of FOXM1 with metastasis. These results suggest that genomic alterations present in human MFH are conserved in the LSL-Kras(G12D); p53(Flox/Flox) mouse model of soft tissue sarcoma and demonstrate the utility of this pre-clinical model.

Species Specificity

Genomics

Humans

Soft Tissue Neoplasms - metabolism

Gene Expression Profiling - methods

Genotype

Histiocytoma, Malignant Fibrous - genetics

Sarcoma - metabolism

Genes, p53

Neoplasm Metastasis

Animals

Histiocytoma, Malignant Fibrous - metabolism

Microarray Analysis

Mice

Genome

Disease Models, Animal

Details

Title: Subtitle: Cross species genomic analysis identifies a mouse model as undifferentiated pleomorphic sarcoma/malignant fibrous histiocytoma
Creators: Jeffrey K Mito - Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States of America
Richard F Riedel
Leslie Dodd
Guy Lahat
Alexander J Lazar
Rebecca D Dodd
Lars Stangenberg
William C Eward
Francis J Hornicek
Sam S Yoon
Brian E Brigman
Tyler Jacks
Dina Lev
Sayan Mukherjee
David G Kirsch
Resource Type: Journal article
Publication Details: PloS one, Vol.4(11), pp.e8075-e8075
DOI: 10.1371/journal.pone.0008075
PMID: 19956606
PMCID: PMC2779485
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Grant note: R01 CA 138265 / NCI NIH HHS Howard Hughes Medical Institute KO8 CA 114176 / NCI NIH HHS T32 GM007171 / NIGMS NIH HHS K08 CA114176 / NCI NIH HHS T32 GM-07171 / NIGMS NIH HHS R01 CA138265 / NCI NIH HHS
Language: English
Date published: 11/30/2009
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984094777602771

Metrics

16 Record Views

59 Times Cited - Web of Science