Journal article
Cryoinsult parameter effects on the histologically apparent volume of experimentally induced osteonecrotic lesions
Journal of orthopaedic research, Vol.29(6), pp.931-937
06/2011
DOI: 10.1002/jor.21342
PMCID: PMC3082588
PMID: 21259339
Abstract
Investigation of femoral head osteonecrosis would benefit from an animal model whose natural history includes progression to bony collapse of a segmental necrotic lesion. The bipedal emu holds attraction for systematic organ-level study of collapse mechanopathology. One established method of experimentally inducing segmental lesions is liquid nitrogen cryoinsult. Four cryoinsult parameters-hold temperature, freeze duration, freeze/thaw repetition, and thaw duration-were investigated to determine their individual and combined effects on resulting necrotic lesion morphology. 3D distributions of histologically apparent osteocyte necrosis from 24 emus receiving varying cryoinsults were used to develop univariate and multivariate linear regression models relating resulting necrotic lesion morphology to particular cryoinsult input parameters. These models were then applied to predict lesion size in four additional emus receiving differing input cryoinsults. The best multivariate regression model predicted lesion volumes that were accurate to better than 8% of overall emu femoral head volume. The hold temperature during cryoinsult was by far the most influential cryoinsult input parameter. The utility of this information is to enhance the consistency and predictability of cryoinsult-induced segmental lesion size for the purposes of systematic laboratory studies at the whole-organ level.
Details
- Title: Subtitle
- Cryoinsult parameter effects on the histologically apparent volume of experimentally induced osteonecrotic lesions
- Creators
- Jessica E Goetz - Department of Orthopaedics & Rehabilitation, University of Iowa, Iowa City, Iowa, USA. jessica-goetz@uiowa.eduDuane A RobinsonDouglas R PedersenMichael G ConzemiusThomas D Brown
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic research, Vol.29(6), pp.931-937
- Publisher
- United States
- DOI
- 10.1002/jor.21342
- PMID
- 21259339
- PMCID
- PMC3082588
- ISSN
- 0736-0266
- eISSN
- 1554-527X
- Grant note
- AR049919 / NIAMS NIH HHS R01 AR049919-04 / NIAMS NIH HHS R01 AR049919 / NIAMS NIH HHS
- Language
- English
- Date published
- 06/2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthopedics and Rehabilitation
- Record Identifier
- 9984040382402771
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