Cryopreservation of Plasmodium Sporozoites

Carson Bowers; Lisa Hancox; Kristen Peissig; Justine C. Shiau; Amélie Vantaux; Benoit Witkowski; Sivchheng Phal; Steven P. Maher; John T. Harty; Dennis E. Kyle; Samarchith P. Kurup

doi:10.3390/pathogens11121487

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Cryopreservation of Plasmodium Sporozoites

Journal article

Open access

Peer reviewed

Cryopreservation of Plasmodium Sporozoites

Carson Bowers, Lisa Hancox, Kristen Peissig, Justine C. Shiau, Amélie Vantaux, Benoit Witkowski, Sivchheng Phal, Steven P. Maher, John T. Harty, Dennis E. Kyle, …

Pathogens (Basel), Vol.11(12), p.1487

12/07/2022

DOI: 10.3390/pathogens11121487

PMCID: PMC9784981

PMID: 36558821

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Abstract

Malaria is a deadly disease caused by the parasite, Plasmodium , and impacts the lives of millions of people around the world. Following inoculation into mammalian hosts by infected mosquitoes, the sporozoite stage of Plasmodium undergoes obligate development in the liver before infecting erythrocytes and causing clinical malaria. The most promising vaccine candidates for malaria rely on the use of attenuated live sporozoites to induce protective immune responses. The scope of widespread testing or clinical use of such vaccines is limited by the absence of efficient, reliable, or transparent strategies for the long-term preservation of live sporozoites. Here we outline a method to cryopreserve the sporozoites of various human and murine Plasmodium species. We found that the structural integrity, viability, and in vivo or in vitro infectiousness were conserved in the recovered cryopreserved sporozoites. Cryopreservation using our approach also retained the transgenic properties of sporozoites and immunization with cryopreserved radiation attenuated sporozoites (RAS) elicited strong immune responses. Our work offers a reliable protocol for the long-term storage and recovery of human and murine Plasmodium sporozoites and lays the groundwork for the widespread use of live sporozoites for research and clinical applications.

Cryopreservation

Malaria

freezing

Plasmodium

RAS vaccination

sporozoite

Details

Title: Subtitle: Cryopreservation of Plasmodium Sporozoites
Creators: Carson Bowers - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA
Lisa Hancox - University of Iowa
Kristen Peissig - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Justine C. Shiau - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Amélie Vantaux - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Benoit Witkowski - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Sivchheng Phal - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Steven P. Maher - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
John T. Harty - University of Iowa
Dennis E. Kyle - Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30605, USA Department of Pathology, University of Iowa, Iowa City, IA 52242, USA Department of Infectious Diseases, University of Georgia, Athens, GA 30605, USA Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh 12201, Cambodia Department of Cellular Biology, University of Georgia, Athens, GA 30605, USA
Samarchith P. Kurup - University of Iowa
Resource Type: Journal article
Publication Details: Pathogens (Basel), Vol.11(12), p.1487
DOI: 10.3390/pathogens11121487
PMID: 36558821
PMCID: PMC9784981
NLM abbreviation: Pathogens
ISSN: 2076-0817
eISSN: 2076-0817
Publisher: MDPI
Grant note: UGA startup funding R21AI144591; T32AI1060546; R01AI167847; 1R01AI168307 / NIH
Language: English
Date published: 12/07/2022
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984353742202771

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