Crystal structure of S-glutathiolated carbonic anhydrase III

Robert J Mallis; Bradley W Poland; Tapan K Chatterjee; Rory A Fisher; Steven Darmawan; Richard B Honzatko; James A Thomas

doi:10.1016/S0014-5793(00)02022-6

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Crystal structure of S-glutathiolated carbonic anhydrase III

Journal article

Open access

Peer reviewed

Crystal structure of S-glutathiolated carbonic anhydrase III

Robert J Mallis, Bradley W Poland, Tapan K Chatterjee, Rory A Fisher, Steven Darmawan, Richard B Honzatko and James A Thomas

FEBS letters, Vol.482(3), pp.237-241

2000

DOI: 10.1016/S0014-5793(00)02022-6

PMID: 11024467

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Abstract

S-Glutathiolation of carbonic anhydrase III (CAIII) occurs rapidly in hepatocytes under oxidative stress. The crystal structure of the S-glutathiolated CAIII from rat liver reveals covalent adducts on cysteines 183 and 188. Electrostatic charge and steric contacts at each modification site inversely correlate with the relative rates of reactivity of these cysteines toward glutathione (GSH). Diffuse electron density associated with the GSH adducts suggests a lack of preferred bonding interactions between CAIII and the glutathionyl moieties. Hence, the GSH adducts are available for binding by a protein capable of reducing this mixed disulfide. These properties are consistent with the participation of CAIII in the protection/recovery from the damaging effects of oxidative agents.

Oxidative Stress

S-Glutathiolation

Protein oxidation

Carbonic anhydrase

Rat liver

Sulfhydryl reactivity

Details

Title: Subtitle: Crystal structure of S-glutathiolated carbonic anhydrase III
Creators: Robert J Mallis - Department of Biochemistry, Biophysics and Molecular Biology, 1210 Molecular Biology Bldg., Iowa State University, Ames, IA 50011, USA
Bradley W Poland - Department of Biochemistry, Biophysics and Molecular Biology, 1210 Molecular Biology Bldg., Iowa State University, Ames, IA 50011, USA
Tapan K Chatterjee - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USA
Rory A Fisher - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USA
Steven Darmawan - Department of Biochemistry, Biophysics and Molecular Biology, 1210 Molecular Biology Bldg., Iowa State University, Ames, IA 50011, USA
Richard B Honzatko - Department of Biochemistry, Biophysics and Molecular Biology, 1210 Molecular Biology Bldg., Iowa State University, Ames, IA 50011, USA
James A Thomas - Department of Biochemistry, Biophysics and Molecular Biology, 1210 Molecular Biology Bldg., Iowa State University, Ames, IA 50011, USA
Resource Type: Journal article
Publication Details: FEBS letters, Vol.482(3), pp.237-241
DOI: 10.1016/S0014-5793(00)02022-6
PMID: 11024467
NLM abbreviation: FEBS Lett
ISSN: 0014-5793
eISSN: 1873-3468
Publisher: Elsevier B.V
Language: English
Date published: 2000
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040310802771

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