Journal article
Crystal structure of adenovirus E3-19K bound to HLA-A2 reveals mechanism for immunomodulation
Nature structural & molecular biology, Vol.19(11), pp.1176-1181
11/01/2012
DOI: 10.1038/nsmb.2396
PMCID: PMC3492506
PMID: 23042604
Abstract
E3-19K binds to and retains MHC class I molecules in the endoplasmic reticulum, suppressing anti-adenovirus activities of T cells. We determined the structure of the adenovirus serotype 2 (Ad2, species C) E3-19K-HLA-A2 complex to 1.95-angstrom resolution. Ad2 E3-19K binds to the N terminus of the HLA-A2 groove, contacting the alpha 1, alpha 2 and alpha 3 domains and beta(2)m. Ad2 E3-19K has a unique structure comprising a large N-terminal domain, formed by two partially overlapping beta-sheets arranged in a V shape, and a C-terminal alpha-helix and tail. The structure reveals determinants in E3-19K and HLA-A2 that are important for complex formation; conservation of some of these determinants in E3-19K proteins of different species and MHC I molecules of different loci suggests a universal binding mode for all E3-19K proteins. Our structure is important for understanding the immunomodulatory function of E3-19K.
Details
- Title: Subtitle
- Crystal structure of adenovirus E3-19K bound to HLA-A2 reveals mechanism for immunomodulation
- Creators
- Lenong Li - University of Illinois ChicagoYasameen Muzahim - University of Illinois ChicagoMarlene Bouvier - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Nature structural & molecular biology, Vol.19(11), pp.1176-1181
- DOI
- 10.1038/nsmb.2396
- PMID
- 23042604
- PMCID
- PMC3492506
- NLM abbreviation
- Nat Struct Mol Biol
- ISSN
- 1545-9993
- eISSN
- 1545-9985
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- R01AI045070 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01 AI045070; R56 AI102468 / US National Institute of Allergy and Infectious Diseases; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Language
- English
- Date published
- 11/01/2012
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984695827902771
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