Crystal structure of the superantigen staphylococcal enterotoxin type A

E M Schad; I Zaitseva; V N Zaitsev; M Dohlsten; T Kalland; P M Schlievert; D H Ohlendorf; L A Svensson

doi:10.1002/j.1460-2075.1995.tb07336.x

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Crystal structure of the superantigen staphylococcal enterotoxin type A

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Crystal structure of the superantigen staphylococcal enterotoxin type A

E M Schad, I Zaitseva, V N Zaitsev, M Dohlsten, T Kalland, P M Schlievert, D H Ohlendorf and L A Svensson

The EMBO journal, Vol.14(14), pp.3292-3301

07/17/1995

DOI: 10.1002/j.1460-2075.1995.tb07336.x

PMCID: PMC394397

PMID: 7628431

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Abstract

Staphylococcal enterotoxins are prototype superantigens characterized by their ability to bind to major histocompatibility complex (MHC) class II molecules and subsequently activate a large fraction of T-lymphocytes. The crystal structure of staphylococcal enterotoxin type A (SEA), a 27 kDa monomeric protein, was determined to 1.9 A resolution with an R-factor of 19.9% by multiple isomorphous replacement. SEA is a two domain protein composed of a beta-barrel and a beta-grasp motif demonstrating the same general structure as staphylococcal enterotoxins SEB and TSST-1. Unique for SEA, however, is a Zn2+ coordination site involved in MHC class II binding. Four amino acids including Ser1, His187, His225 and Asp227 were found to be involved in direct coordination of the metal ion. SEA is the first Zn2+ binding enterotoxin that has been structurally determined.

Details

Title: Subtitle: Crystal structure of the superantigen staphylococcal enterotoxin type A
Creators: E M Schad - Molecular Biophysics, Chemical Centre, Lund University
I Zaitseva - Molecular Biophysics, Chemical Centre, Lund University
V N Zaitsev - Molecular Biophysics, Chemical Centre, Lund University
M Dohlsten - Molecular Biophysics, Chemical Centre, Lund University
T Kalland - Molecular Biophysics, Chemical Centre, Lund University
P M Schlievert - Molecular Biophysics, Chemical Centre, Lund University
D H Ohlendorf - Molecular Biophysics, Chemical Centre, Lund University
L A Svensson - Molecular Biophysics, Chemical Centre, Lund University
Resource Type: Journal article
Publication Details: The EMBO journal, Vol.14(14), pp.3292-3301
DOI: 10.1002/j.1460-2075.1995.tb07336.x
PMID: 7628431
PMCID: PMC394397
ISSN: 0261-4189
eISSN: 1460-2075
Language: English
Date published: 07/17/1995
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002378702771

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