Crystallization and preliminary X-ray diffraction analysis of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB 12038

Zulfiqar A Malik; Christopher C R Allen; Lokesh Gakhar; David A Lipscomb; Michael J Larkin; S Ramaswamy

doi:10.1107/S0907444902016803

$Crystallization and preliminary X-ray diffraction analysis of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB 12038$

Journal article

Peer reviewed

Crystallization and preliminary X-ray diffraction analysis of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB 12038

Zulfiqar A Malik, Christopher C R Allen, Lokesh Gakhar, David A Lipscomb, Michael J Larkin and S Ramaswamy

Acta crystallographica. Section D, Biological crystallography, Vol.58(Pt 12), pp.2173-2174

12/2002

DOI: 10.1107/S0907444902016803

PMID: 12454491

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Abstract

The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene by Rhodococcus sp. strain NCIMB 12038. The terminal oxygenase component (naphthalene 1,2-dioxygenase) that catalyzes this reaction belongs to the aromatic ring hydroxylating dioxygenase family and has been crystallized. These enzymes utilize a mononuclear non-heme iron centre to catalyze the addition of dioxygen to their respective substrates. In this reaction, two electrons, two protons and a dioxygen molecule are consumed. The Rhodococcus enzyme has only 33 and 29% sequence identity to the corresponding alpha- and beta-subunits of the NDO system of Pseudomonas putida NCIMB 9816-4, for which the tertiary structure has been reported. In order to determine the three-dimensional structure of the Rhodococcus NDO, diffraction-quality crystals have been prepared by the hanging-drop method. The crystals belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 87.5, b = 144, c = 185.6 A, alpha = beta = gamma = 90 degrees, and diffract to 2.3 A resolution.

Crystallization

Crystallography, X-Ray

Dioxygenases

Multienzyme Complexes - chemistry

Oxygenases - chemistry

Protein Conformation

Rhodococcus - enzymology

Details

Title: Subtitle: Crystallization and preliminary X-ray diffraction analysis of naphthalene dioxygenase from Rhodococcus sp. strain NCIMB 12038
Creators: Zulfiqar A Malik - University of Iowa
Christopher C R Allen
Lokesh Gakhar - University of Iowa
David A Lipscomb
Michael J Larkin
S Ramaswamy - University of Iowa
Resource Type: Journal article
Publication Details: Acta crystallographica. Section D, Biological crystallography, Vol.58(Pt 12), pp.2173-2174
DOI: 10.1107/S0907444902016803
PMID: 12454491
ISSN: 0907-4449
eISSN: 1399-0047
Grant note: GM062904-01A1 / NIGMS NIH HHS
Language: English
Date published: 12/2002
Academic Unit: Medicine Administration
Record Identifier: 9984622752402771

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