Journal article
Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest
Journal of oncology, Vol.2012, 601682
2012
DOI: 10.1155/2012/601682
PMCID: PMC3368438
PMID: 22690217
Abstract
Purpose. To explore the effects of cucurbitacin B on the radiation survival of human breast cancer cells and to elucidate the cellular mechanism of radiosensitization if any.Materials and Methods. Human breast carcinoma cell lines were treated with cucurbitacin B before irradiation with 0–10 Gy ofC137sgamma rays. The effect of cucurbitacin B on cell-survival following irradiation was evaluated by colony-forming assay. Cell cycle distributions were investigated using flow cytometry. Real-time PCR and western blots were performed to investigate the expression of cell cycle checkpoints.Results.Cucurbitacin B inhibited breast cancer cell proliferation in a dose-dependent manner. Only MDA-MB-231 and MCF7:5C cells but not SKBR-3 cells were radiosensitized by cucurbitacin B. Flow cytometric analysis for DNA content indicated that cucurbitacin B resulted in G2/M arrest in MDA-MB-231 and MCF7:5C but not SKBR-3 cells. Moreover, Real-time PCR and western blot analysis demonstrated upregulated p21 expression before irradiation, a likely cause of the cell cycle arrest.Conclusion. Taken together, these findings suggest that cucurbitacin B causes radiosensitization of some breast cancer cells, and that cucurbitacin B induced G2/M arrest is an important mechanism. Therefore, combinations of cucurbitacin B with radiotherapy may be appropriate for experimental breast cancer treatment.Corrigendum to “Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest”
Details
- Title: Subtitle
- Cucurbitacin B Causes Increased Radiation Sensitivity of Human Breast Cancer Cells via G2/M Cell Cycle Arrest
- Creators
- Suwit Duangmano - University of IowaPhorntip Sae-lim - Faculty of Science, Ramkhamhaeng University, Bangkok, ThailandApichart Suksamrarn - Faculty of Science, Ramkhamhaeng University, Bangkok, ThailandPimpicha Patmasiriwat - Faculty of Medical Technology, Mahidol University, Bangkok, ThailandFrederick E Domann - Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of oncology, Vol.2012, 601682
- DOI
- 10.1155/2012/601682
- PMID
- 22690217
- PMCID
- PMC3368438
- NLM abbreviation
- J Oncol
- ISSN
- 1687-8450
- eISSN
- 1687-8450
- Grant note
- DOI: 10.13039/100000054, name: National Cancer Institute, award: P30 CA086862
- Language
- English
- Date published
- 2012
- Academic Unit
- Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984047675702771
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