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Cultures of Diabetic Foot Ulcers Without Clinical Signs of Infection Do Not Predict Outcomes
Journal article   Open access   Peer reviewed

Cultures of Diabetic Foot Ulcers Without Clinical Signs of Infection Do Not Predict Outcomes

Sue E Gardner, Ambar Haleem, Ying-Ling Jao, Stephen L Hillis, John E Femino, Phinit Phisitkul, Kristopher P Heilmann, Shannon M Lehman and Carrie L Franciscus
Diabetes Care, Vol.37(10), pp.2693-2701
0
10/2014
DOI: 10.2337/dc14-0051
PMCID: PMC4170124
PMID: 25011945
url
https://europepmc.org/articles/pmc4170124View
Published (Version of record) Open Access

Abstract

OBJECTIVE We examined associations between ulcer bioburden and ulcer outcomes in neuropathic diabetic foot ulcers (DFUs) that lacked clinical signs of infection. RESEARCH DESIGN AND METHODS Three dimensions of bioburden (i.e., microbial load, microbial diversity, and the presence of likely pathogens) were measured at baseline using swab cultures obtained by Levine’s technique. Subjects were assessed every 2 weeks for 26 weeks to determine the rate of healing and development of infection-related complications. Foot ulcers were off-loaded using total-contact casts and routinely debrided. To establish associations between bioburden and rate of healing, Cox proportional hazards and least squares regression were used after adjusting for ulcer depth, surface area, and duration. RESULTS A total of 77 subjects completed the study. Sixty-five (84.4%) had ulcers that healed during follow-up; weeks-to-closure ranged from 2 to 26 (median 4.0). Mean (± SD) percent reduction in surface area/week was 25.0% (± 23.33). Five (6.5%) of the DFUs developed an infection-related complication. None of the bioburden dimensions (i.e., microbial load, microbial diversity, or presence of likely pathogens) was significantly associated with weeks-to-closure or percent reduction in surface area per week. Weeks-to-closure was best predicted by ulcer duration, depth, and surface area (c-statistic = 0.75). CONCLUSIONS Culturing DFUs that showed no clinical signs of infection had no predictive value for outcomes of DFUs managed with total-contact casts and routine debridement. These findings support recommendations of the Infectious Disease Society of America that culturing and antibiotics should be avoided in treating DFUs that show no clinical signs of infection.
Clinical Care/Education/Nutrition/Psychosocial Research

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