Journal article
Curcumin and Anthocyanin Inhibit Pepsin-Mediated Cell Damage and Carcinogenic Changes in Airway Epithelial Cells
Annals of otology, rhinology & laryngology, Vol.122(10), pp.632-641
10/2013
DOI: 10.1177/000348941312201006
PMID: 24294686
Abstract
Objectives: Laryngopharyngeal reflux (LPR) is associated with inflammatory and neoplastic airway diseases. Gastric pepsin internalized by airway epithelial cells during reflux contributes to oxidative stress, inflammation, and carcinogenesis. Several plant extracts and compounds inhibit digestive enzymes and inflammatory or neoplastic changes to the esophagus in models of gastroesophageal reflux. This study examined the potential of chemoprotective phytochemicals to inhibit peptic activity and mitigate pepsin-mediated damage of airway epithelial cells.
Methods: Cultured human laryngeal and hypopharyngeal epithelial cells were pretreated with curcumin (10 micromol/L), ecabet sodium (125 microg/mL), and anthocyanin-enriched black-raspberry extract (100 microg/mL) 30 minutes before treatment with pepsin (0.1 mg/mL; 1 hour; pH 7). Controls were treated with media pH 7 or pepsin pH 7 without phytochemicals. Cell damage and proliferative changes were assessed by electron microscopy, cell count, thymidine analog incorporation, and real-time polymerase chain reaction array. Pepsin inhibition was determined by in vitro kinetic assay.
Results: Micromolar concentrations of curcumin, ecabet sodium, and black-raspberry extract inhibited peptic activity and pepsin-induced mitochondrial damage and hyperproliferation. Curcumin abrogated pepsin-mediated depression of tumor suppressor gene expression and altered the subcellular localization of pepsin following endocytosis.
Conclusions: Several phytochemicals inhibit the pepsin-mediated cell damage underlying inflammatory or neoplastic manifestations of LPR. Dietary supplementation or adjunctive therapy with phytochemicals may represent novel preventive or therapeutic strategies for LPR-attributed disease.
Details
- Title: Subtitle
- Curcumin and Anthocyanin Inhibit Pepsin-Mediated Cell Damage and Carcinogenic Changes in Airway Epithelial Cells
- Creators
- Tina L Samuels - Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, WisconsinAmy C. S Pearson - Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, WisconsinClive W Wells - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WisconsinGary D Stoner - Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin., Cancer Center, Medical College of Wisconsin, Milwaukee, WisconsinNikki Johnston - Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.122(10), pp.632-641
- DOI
- 10.1177/000348941312201006
- PMID
- 24294686
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Anesthesia
- Record Identifier
- 9984007194202771
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