Cut mutant Drosophila auditory organs differentiate abnormally and degenerate

Dominic J S Ebacher; Sokol V Todi; Daniel F Eberl; Grace E Boekhoff-Falk

doi:10.4161/fly.4242

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Cut mutant Drosophila auditory organs differentiate abnormally and degenerate

Journal article

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Cut mutant Drosophila auditory organs differentiate abnormally and degenerate

Dominic J S Ebacher, Sokol V Todi, Daniel F Eberl and Grace E Boekhoff-Falk

Fly (Austin, Tex.), Vol.1(2), pp.86-94

03/2007

DOI: 10.4161/fly.4242

PMCID: PMC4309998

PMID: 18820445

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Abstract

The Drosophila antenna is a sophisticated structure that functions in both olfaction and audition. Previous studies have identified Homothorax, Extradenticle, and Distal-less, three homeodomain transcription factors, as required for specification of antennal identity. Antennal expression of cut is activated by Homothorax and Extradenticle, and repressed by Distal-less. cut encodes the Drosophila homolog of human CAAT-displacement protein, a cell cycle-regulated homeodomain transcription factor. Cut is required for normal development of external mechanosensory structures and Malphigian tubules (kidney analogs). The role of cut in the Drosophila auditory organ, Johnston's organ, has not been characterized. We have employed the FLP/FRT system to generate cut null clones in developing Johnston's organ. In cut mutants, the scolopidial subunits that constitute Johnston's organ differentiate abnormally and subsequently degenerate. Electrophysiological experiments confirm that adult Drosophila with cut null antennae are deaf. We find that cut acts in parallel to atonal, spalt-major, and spalt-related, which encode other transcription factors required for Johnston's organ differentiation. We speculate that Cut functions in conjunction with these factors to regulate transcription of as yet unidentified targets.

Deafness - genetics

Neurons

Homeodomain Proteins - metabolism

Nuclear Proteins - metabolism

Transcription Factors - genetics

Drosophila Proteins - metabolism

Drosophila melanogaster - genetics

Homeodomain Proteins - genetics

Transcription Factors - metabolism

Integumentary System - growth & development

Animals

Sense Organs - growth & development

Drosophila melanogaster - growth & development

Sense Organs - metabolism

Drosophila Proteins - genetics

Mutation

Nuclear Proteins - genetics

Gene Expression Regulation, Developmental - physiology

Details

Title: Subtitle: Cut mutant Drosophila auditory organs differentiate abnormally and degenerate
Creators: Dominic J S Ebacher - Department of Anatomy, University of Wisconsin, Madison, Wisconsin 53706, USA
Sokol V Todi
Daniel F Eberl
Grace E Boekhoff-Falk
Resource Type: Journal article
Publication Details: Fly (Austin, Tex.), Vol.1(2), pp.86-94
Publisher: United States
DOI: 10.4161/fly.4242
PMID: 18820445
PMCID: PMC4309998
ISSN: 1933-6934
eISSN: 1933-6942
Grant note: GM59871 / NIGMS NIH HHS R01 DC004848-07 / NIDCD NIH HHS RR12294 / NCRR NIH HHS T32 GM07133 / NIGMS NIH HHS R01 DC004848-05 / NIDCD NIH HHS R01 RR012294 / NCRR NIH HHS DC04848 / NIDCD NIH HHS R01 DC004848 / NIDCD NIH HHS R01 DC004848-04 / NIDCD NIH HHS R01 GM059871 / NIGMS NIH HHS R01 DC004848-06 / NIDCD NIH HHS T32 GM007133 / NIGMS NIH HHS
Language: English
Date published: 03/2007
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984070397202771

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