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Cutting Edge: Antilisterial Activity of CD8+T Cells Derived from TNF-Deficient and TNF/Perforin Double-Deficient Mice
Journal article   Peer reviewed

Cutting Edge: Antilisterial Activity of CD8+T Cells Derived from TNF-Deficient and TNF/Perforin Double-Deficient Mice

Douglas W White, Vladimir P Badovinac, George Kollias and John T Harty
The Journal of immunology (1950), Vol.165(1), pp.5-9
07/01/2000
DOI: 10.4049/jimmunol.165.1.5
PMID: 10861027

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Abstract

The mechanisms by which CD8+ T cells mediate immunity against bacterial pathogens remain largely unknown. Perforin-dependent cytolysis plays a role, but is not required for CD8+ T cell-mediated immunity against Listeria monocytogenes. TNF is essential for CD8+ T cell immunity to L. monocytogenes, but the cellular source of TNF is undefined. TNF-deficient and TNF/perforin double-deficient mice were used to generate CD8+ T cells specific for an L. monocytogenes-derived Ag. Wild-type and TNF-deficient CD8+ T cells mediated antilisterial immunity in wild-type but not TNF-deficient host mice, revealing that CD8+ T cell-derived TNF is not required for CD8+ T cell-mediated antilisterial immunity, but demonstrating a role for TNF derived from other cell types. TNF/perforin double-deficient CD8+ T cells mediated antilisterial immunity in the liver, but not in the spleen, of wild-type recipient mice, suggesting that perforin-independent immunity in the spleen requires CD8+ T cell-derived TNF.

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