Journal article
Cutting Edge: Detection of a High Frequency of Virus-Specific CD4+ T Cells During Acute Infection with Lymphocytic Choriomeningitis Virus
The Journal of immunology (1950), Vol.161(7), pp.3215-3218
10/01/1998
DOI: 10.4049/jimmunol.161.7.3215
PMID: 9759834
Abstract
Lymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections.
Details
- Title: Subtitle
- Cutting Edge: Detection of a High Frequency of Virus-Specific CD4+ T Cells During Acute Infection with Lymphocytic Choriomeningitis Virus
- Creators
- Steven M VargaRaymond M Welsh
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.161(7), pp.3215-3218
- DOI
- 10.4049/jimmunol.161.7.3215
- PMID
- 9759834
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Am Assoc Immnol
- Language
- English
- Date published
- 10/01/1998
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083293102771
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