Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes

Tony J Vanden Bush; Claire M Buchta; Jennifer Claudio; Gail A Bishop

doi:10.4049/jimmunol.0900968

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Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes

Journal article

Peer reviewed

Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes

Tony J Vanden Bush, Claire M Buchta, Jennifer Claudio and Gail A Bishop

The Journal of immunology (1950), Vol.183(8), pp.4833-4837

10/15/2009

DOI: 10.4049/jimmunol.0900968

PMID: 19801508

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Abstract

B lymphocytes are a potential alternative to dendritic cell immunotherapy, with the advantages of relative abundance in peripheral blood and the ability to function as APCs. Although B cells express multiple receptors that induce costimulatory molecules, B cell vaccine studies have focused primarily on CD40 stimulation. To optimize the potential efficacy of B cell vaccines (Bvac), we compared the capacity of differentially stimulated B cells to induce Ag-specific CD8(+) T cell responses in vivo. CD40- or TLR7-stimulated B cell APCs induced similar CD8(+) T cell responses, but costimulation through the BCR and TLR7 produced a more effective Bvac as measured by T cell stimulation and the protection of mice from an infectious pathogen. This increased effectiveness depended upon enhanced production of IL-6 by BCR plus TLR7-stimulated B cells. These findings reveal alternative stimulation strategies for the production of effective Bvac and identify a key role for IL-6 in B cell Ag presentation and cellular vaccines.

Tumor Necrosis Factor-alpha - metabolism

B-Lymphocytes - microbiology

CD8-Positive T-Lymphocytes - microbiology

Ovalbumin - immunology

Mice, Inbred C57BL

Vaccination

Vaccines - immunology

Listeria monocytogenes - immunology

Receptors, Antigen, B-Cell - metabolism

Receptors, Antigen, B-Cell - immunology

Lymphocyte Activation - immunology

Animals

B-Lymphocytes - immunology

Interleukin-6 - pharmacology

CD8-Positive T-Lymphocytes - drug effects

Toll-Like Receptor 7 - immunology

Interleukin-6 - immunology

Tumor Necrosis Factor-alpha - immunology

Mice

Membrane Glycoproteins - immunology

CD8-Positive T-Lymphocytes - immunology

Interleukin-6 - metabolism

B-Lymphocytes - transplantation

Details

Title: Subtitle: Cutting Edge: Importance of IL-6 and cooperation between innate and adaptive immune receptors in cellular vaccination with B lymphocytes
Creators: Tony J Vanden Bush - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Claire M Buchta
Jennifer Claudio
Gail A Bishop
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.183(8), pp.4833-4837
DOI: 10.4049/jimmunol.0900968
PMID: 19801508
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Language: English
Date published: 10/15/2009
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984001215002771

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