Cutting edge: CD8 T cell-mediated demyelination is IFN-gamma dependent in mice infected with a neurotropic coronavirus

Lecia Pewe; Stanley Perlman

doi:10.4049/jimmunol.168.4.1547

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Cutting edge: CD8 T cell-mediated demyelination is IFN-gamma dependent in mice infected with a neurotropic coronavirus

Journal article

Peer reviewed

Cutting edge: CD8 T cell-mediated demyelination is IFN-gamma dependent in mice infected with a neurotropic coronavirus

Lecia Pewe and Stanley Perlman

Journal of immunology (Baltimore, Md. : 1950), Vol.168(4), pp.1547-1551

02/15/2002

DOI: 10.4049/jimmunol.168.4.1547

PMID: 11823480

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Abstract

Mice infected with the murine coronavirus, mouse hepatitis virus, strain JHM (MHV) develop an immune-mediated demyelinating encephalomyelitis. We showed previously that adoptive transfer of MHV-immune splenocytes depleted of either CD4 or CD8 T cells to infected RAG1(-/-) recipients (mice deficient in recombination activation gene 1) resulted in demyelination. Herein we show that transfer of CD8 T cell-enriched splenocytes from MHV-immune IFN-gamma(-/-) donors resulted in a substantial decrease in demyelination (4.8% of the white matter of the spinal cord compared with 26.3% in those receiving cells from C57BL/6 donors). Similar numbers of lymphocytes were present in the CNS of recipients of either C57BL/6 or IFN-gamma(-/-) CD8 T cells, suggesting that IFN-gamma was not crucial for lymphocyte entry into the CNS. Rather, IFN-gamma was critical for optimal activation or migration of macrophages or microglia into the white matter in the context of CD8 T cell-mediated demyelination.

Encephalomyelitis, Autoimmune, Experimental - pathology

Spleen - immunology

Myelin Sheath - pathology

CD8-Positive T-Lymphocytes - transplantation

Mice, Inbred C57BL

Encephalomyelitis, Autoimmune, Experimental - immunology

Interferon-gamma - physiology

Adoptive Transfer

Coronavirus Infections - immunology

Encephalomyelitis, Autoimmune, Experimental - virology

Mice, Knockout

Murine hepatitis virus - isolation & purification

Animals

Microglia - immunology

Coronavirus Infections - virology

Spinal Cord - pathology

Coronavirus Infections - pathology

Mice

Interferon-gamma - genetics

CD8-Positive T-Lymphocytes - immunology

Macrophages - immunology

Cell Movement

Details

Title: Subtitle: Cutting edge: CD8 T cell-mediated demyelination is IFN-gamma dependent in mice infected with a neurotropic coronavirus
Creators: Lecia Pewe - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of immunology (Baltimore, Md. : 1950), Vol.168(4), pp.1547-1551
DOI: 10.4049/jimmunol.168.4.1547
PMID: 11823480
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: NS40438-01 / NINDS NIH HHS
Language: English
Date published: 02/15/2002
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777476702771

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