Cutting edge: contrasting roles of TNF receptor-associated factor 2 (TRAF2) and TRAF3 in CD40-activated B lymphocyte differentiation

B S Hostager; G A Bishop

doi:10.4049/jimmunol.162.11.6307

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Cutting edge: contrasting roles of TNF receptor-associated factor 2 (TRAF2) and TRAF3 in CD40-activated B lymphocyte differentiation

Journal article

Peer reviewed

Cutting edge: contrasting roles of TNF receptor-associated factor 2 (TRAF2) and TRAF3 in CD40-activated B lymphocyte differentiation

B S Hostager and G A Bishop

The Journal of immunology (1950), Vol.162(11), pp.6307-6311

06/01/1999

DOI: 10.4049/jimmunol.162.11.6307

PMID: 10352240

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Abstract

In B lymphocytes, CD40 signals contribute to the activation of Ab secretion, isotype switching, T cell costimulation, and immunological memory. TRAF proteins appear to be important components of the CD40 signal transduction complex, but their roles in the activation of B cell effector functions are poorly understood. We examined the contributions of TNF receptor-associated factor 2 (TRAF2) and TRAF3 to CD40-activated differentiation in mouse B cells transfected with inducible TRAF and dominant-negative TRAF cDNAs. We find that binding of TRAF2 and TRAF3 to CD40 is not required for the induction of Ab secretion, but that both TRAF molecules can regulate the activation process. We demonstrate a negative regulatory role for TRAF3 and that this activity is dependent on the availability of an intact TRAF3-binding site in the cytoplasmic domain of CD40. In contrast, TRAF2 appears to play a positive role in B cell differentiation, and this activity is apparent even when its binding site on CD40 is disrupted.

Protein Biosynthesis

Humans

Molecular Sequence Data

Transfection - immunology

Receptors, Tumor Necrosis Factor - physiology

TNF Receptor-Associated Factor 2

TNF Receptor-Associated Factor 3

Cell Differentiation - genetics

B-Lymphocytes - secretion

CD40 Antigens - metabolism

CD40 Antigens - genetics

Tumor Cells, Cultured

Amino Acid Sequence

CD40 Antigens - physiology

Cell Line

Proteins - physiology

Antibody Formation - immunology

B-Lymphocytes - cytology

Lymphocyte Activation

Protein Binding - immunology

Proteins - genetics

Cell Differentiation - immunology

Animals

B-Lymphocytes - immunology

Proteins - metabolism

Mice

Antibody Formation - genetics

Details

Title: Subtitle: Cutting edge: contrasting roles of TNF receptor-associated factor 2 (TRAF2) and TRAF3 in CD40-activated B lymphocyte differentiation
Creators: B S Hostager - Department of Microbiology, University of Iowa, VA Medical Center, Iowa City 52242, USA
G A Bishop
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.162(11), pp.6307-6311
Publisher: United States
DOI: 10.4049/jimmunol.162.11.6307
PMID: 10352240
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI28847 / NIAID NIH HHS CA66570 / NCI NIH HHS DK25295 / NIDDK NIH HHS
Language: English
Date published: 06/01/1999
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984001224002771

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