Cutting edge: contribution of lung-resident T cell proliferation to the overall magnitude of the antigen-specific CD8 T cell response in the lungs following murine influenza virus infection

Jodi McGill; Kevin L Legge

doi:10.4049/jimmunol.0901109

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Cutting edge: contribution of lung-resident T cell proliferation to the overall magnitude of the antigen-specific CD8 T cell response in the lungs following murine influenza virus infection

Journal article

Peer reviewed

Cutting edge: contribution of lung-resident T cell proliferation to the overall magnitude of the antigen-specific CD8 T cell response in the lungs following murine influenza virus infection

Jodi McGill and Kevin L Legge

The Journal of immunology (1950), Vol.183(7), pp.4177-4181

10/01/2009

DOI: 10.4049/jimmunol.0901109

PMCID: PMC2762786

PMID: 19767567

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Abstract

Following influenza virus infection, CD8 T cells encounter mature, Ag-bearing dendritic cells within the draining lymph nodes and undergo activation, programmed proliferation, and differentiation to effector cells before migrating to the lungs to mediate viral clearance. However, it remains unclear whether CD8 T cells continue their proliferation after arriving in the lungs. To address this question, we developed a novel, in vivo, dual-label system using intranasal CFSE and BrdU administration to identify virus-specific CD8 T cells that are actively undergoing cell division while in the lungs. With this technique we demonstrate that a high frequency of virus-specific CD8 T cells incorporate BrdU while in the lungs and that this lung-resident proliferation contributes significantly to the magnitude of the Ag-specific CD8 T cell response following influenza virus infection.

Influenza A Virus, H1N1 Subtype - immunology

Propylene Glycols - pharmacology

Lung - pathology

Cell Proliferation

Orthomyxoviridae Infections - pathology

Fingolimod Hydrochloride

Cell Movement - immunology

Sphingosine - pharmacology

Lung - virology

Sphingosine - analogs & derivatives

Animals

CD8-Positive T-Lymphocytes - virology

CD8-Positive T-Lymphocytes - metabolism

Female

Mice

Mice, Inbred BALB C

Epitopes, T-Lymphocyte - immunology

CD8-Positive T-Lymphocytes - immunology

Orthomyxoviridae Infections - virology

Lung - immunology

Orthomyxoviridae Infections - immunology

Details

Title: Subtitle: Cutting edge: contribution of lung-resident T cell proliferation to the overall magnitude of the antigen-specific CD8 T cell response in the lungs following murine influenza virus infection
Creators: Jodi McGill - Department of Pathology and Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242, USA
Kevin L Legge
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.183(7), pp.4177-4181
DOI: 10.4049/jimmunol.0901109
PMID: 19767567
PMCID: PMC2762786
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: Oxford University Press (OUP); United States
Grant note: R01 AI071085 / NIAID NIH HHS R21 AI076989-01 / NIAID NIH HHS AI-071085 / NIAID NIH HHS R01 AI071085-03 / NIAID NIH HHS R21 AI076989 / NIAID NIH HHS AI-076989 / NIAID NIH HHS R21 AI076989-02 / NIAID NIH HHS R01 AI071085-01A1 / NIAID NIH HHS R01 AI071085-02 / NIAID NIH HHS
Language: English
Date published: 10/01/2009
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984046823102771

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