Cutting edge: differential segregation of the SRC homology 2-containing protein tyrosine phosphatase-1 within the early NK cell immune synapse distinguishes noncytolytic from cytolytic interactions

Yatin M Vyas; Hina Maniar; Bo Dupont

doi:10.4049/jimmunol.168.7.3150

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Cutting edge: differential segregation of the SRC homology 2-containing protein tyrosine phosphatase-1 within the early NK cell immune synapse distinguishes noncytolytic from cytolytic interactions

Journal article

Peer reviewed

Cutting edge: differential segregation of the SRC homology 2-containing protein tyrosine phosphatase-1 within the early NK cell immune synapse distinguishes noncytolytic from cytolytic interactions

Yatin M Vyas, Hina Maniar and Bo Dupont

The Journal of immunology (1950), Vol.168(7), pp.3150-3154

04/01/2002

DOI: 10.4049/jimmunol.168.7.3150

PMID: 11907066

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Abstract

Inhibitory NK receptors with ligand specificity for MHC class I recruit Src homology 2-containing protein tyrosine phosphatase-1 (SHP-1) phosphatase and prevent autocytotoxicity. Activation of SHP-1 depends upon Src kinase-mediated tyrosine phosphorylation of the cytoplasmic domain of the inhibitory receptor. In this study we demonstrate, by quantitative temporal analysis, that talin, Lck, and SHP-1 are recruited to the synapse within 1 min in both cytolytic and noncytolytic conjugates. Polarization of talin and Lck rapidly disappears in the noncytolytic interactions but persists in cytolytic interactions, where protein kinase C-theta;, Src homology 2 domain-containing leukocyte protein of 76 kDa, and lysosomes are recruited within 5 min. At 1 min SHP-1 clusters in the periphery of the cytolytic synapse, whereas it clusters in the center of the noncytolytic synapse. Lck has multifocal distribution in both synapses consistent with the shared requirement for early tyrosine phosphorylation. Our studies indicate that the spatial location of SHP-1 in the synapse distinguishes noncytolytic from cytolytic interactions within the first minute.

Cell Communication - immunology

Cytotoxicity Tests, Immunologic

Humans

Protein Kinase C-theta

Intracellular Signaling Peptides and Proteins

Lysosomes - enzymology

Protein Tyrosine Phosphatases - metabolism

Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism

Phosphoproteins - metabolism

Talin - metabolism

Killer Cells, Natural - enzymology

Protein Transport - immunology

Signal Transduction - immunology

Adaptor Proteins, Signal Transducing

Isoenzymes - metabolism

Protein Kinase C - metabolism

Killer Cells, Natural - immunology

Clone Cells

src Homology Domains - immunology

Lysosomes - immunology

Protein Phosphatase 1

Cell Line, Transformed

Protein Tyrosine Phosphatase, Non-Receptor Type 6

Cytotoxicity, Immunologic

Details

Title: Subtitle: Cutting edge: differential segregation of the SRC homology 2-containing protein tyrosine phosphatase-1 within the early NK cell immune synapse distinguishes noncytolytic from cytolytic interactions
Creators: Yatin M Vyas - Immunology Program, Sloan-Kettering Institute for Cancer Research, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
Hina Maniar
Bo Dupont
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.168(7), pp.3150-3154
DOI: 10.4049/jimmunol.168.7.3150
PMID: 11907066
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: CA08748 / NCI NIH HHS CA23766 / NCI NIH HHS
Language: English
Date published: 04/01/2002
Academic Unit: Stead Family Department of Pediatrics
Record Identifier: 9984093607302771

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