Journal article
Cyanidin-3-Glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2
Molecular cancer, Vol.9(1), pp.285-285
10/29/2010
DOI: 10.1186/1476-4598-9-285
PMCID: PMC2984473
PMID: 21034468
Abstract
Background: Ethanol is a tumor promoter. Both epidemiological and experimental studies suggest that ethanol may enhance the metastasis of breast cancer cells. We have previously demonstrated that ethanol increased the migration/invasion of breast cancer cells expressing high levels of ErbB2. Amplification of ErbB2 is found in 20-30% of breast cancer patients and is associated with poor prognosis. We sought to identify agents that can prevent or ameliorate ethanol-induced invasion of breast cancer cells. Cyanidin-3-glucoside (C3G), an anthocyanin present in many vegetables and fruits, is a potent natural antioxidant. Ethanol exposure causes the accumulation of intracellular reactive oxygen species (ROS). This study evaluated the effect of C3G on ethanol-induced breast cancer cell migration/invasion.
Results: C3G attenuated ethanol-induced migration/invasion of breast cancer cells expressing high levels of ErbB2 (BT474, MDA-MB231 and MCF7(ErbB2)) in a concentration dependent manner. C3G decreased ethanol-mediated cell adhesion to the extracellular matrix (ECM) as well as the amount of focal adhesions and the formation of lamellipodial protrusion. It inhibited ethanol-stimulated phosphorylation of ErbB2, cSrc, FAK and p130(Cas), as well as interactions among these proteins. C3G abolished ethanol-mediated p130(Cas)/JNK interaction.
Conclusions: C3G blocks ethanol-induced activation of the ErbB2/cSrc/FAK pathway which is necessary for cell migration/invasion. C3G may be beneficial in preventing/reducing ethanol-induced breast cancer metastasis.
Details
- Title: Subtitle
- Cyanidin-3-Glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2
- Creators
- Mei Xu - University of KentuckyKimberly A Bower - University of KentuckySiying Wang - University of KentuckyJacqueline A Frank - University of KentuckyGang Chen - University of KentuckyMin Ding - National Institute for Occupational Safety and HealthShiow Wang - United States Department of AgricultureXianglin Shi - University of KentuckyZunji Ke - Chinese Academy of SciencesJia Luo - University of Kentucky
- Resource Type
- Journal article
- Publication Details
- Molecular cancer, Vol.9(1), pp.285-285
- DOI
- 10.1186/1476-4598-9-285
- PMID
- 21034468
- PMCID
- PMC2984473
- NLM abbreviation
- Mol Cancer
- ISSN
- 1476-4598
- eISSN
- 1476-4598
- Publisher
- BioMed Central
- Language
- English
- Date published
- 10/29/2010
- Academic Unit
- Pathology
- Record Identifier
- 9984186532702771
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