Journal article
Cyclin K Functions as a CDK9 Regulatory Subunit and Participates in RNA Polymerase II Transcription
The Journal of biological chemistry, Vol.274(49), pp.34527-34530
12/03/1999
DOI: 10.1074/jbc.274.49.34527
PMID: 10574912
Abstract
Important progress in the understanding of elongation control by RNA polymerase II (RNAPII) has come from the recent identification of the positive transcription elongation factor b (P-TEFb) and the demonstration that this factor is a protein kinase that phosphorylates the carboxyl-terminal domain (CTD) of the RNAPII largest subunit. The P-TEFb complex isolated from mammalian cells contains a catalytic subunit (CDK9), a cyclin subunit (cyclin T1 or cyclin T2), and additional, yet unidentified, polypeptides of unknown function. To identify additional factors involved in P-TEFb function we performed a yeast two-hybrid screen using CDK9 as bait and found that cyclin K interacts with CDK9 in vivo. Biochemical analyses indicate that cyclin K functions as a regulatory subunit of CDK9. The CDK9-cyclin K complex phosphorylated the CTD of RNAPII and functionally substituted for P-TEFb comprised of CDK9 and cyclin T in in vitro transcription reactions.
Details
- Title: Subtitle
- Cyclin K Functions as a CDK9 Regulatory Subunit and Participates in RNA Polymerase II Transcription
- Creators
- Tsu-Ju FuJunmin PengGary LeeDavid H PriceOsvaldo Flores
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.274(49), pp.34527-34530
- DOI
- 10.1074/jbc.274.49.34527
- PMID
- 10574912
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 12/03/1999
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024503902771
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