Journal article
Cyclosporin A inhibits activation of inducible nitric oxide synthase in C6 glioma cell line
Brain research, Vol.816(1), pp.92-98
1999
DOI: 10.1016/S0006-8993(98)01130-5
PMID: 9878697
Abstract
The effects of immunosuppressant cyclosporin A (CsA) on nitric oxide (NO) production and inducible NO synthase (iNOS) mRNA expression in rat C6 glioma cell line were investigated. CsA applied simultaneously with iNOS activator IFN-γ caused dose-dependent reduction of NO synthesis in confluent C6 cells, as determined by measuring accumulation of nitrite, an indicator of NO production, in 48 h culture supernatants. IFN-γ-induced expression of iNOS, but not interferon regulatory factor-1 (IRF-1) mRNA was reduced in CsA-treated cells. The enzymatic activity of iNOS was not changed by CsA, since it failed to affect NO production in cells in which iNOS had already been induced with IFN-γ and any further induction was blocked by protein synthesis inhibitor cycloheximide (CHX). FK506 was not able to mimic inhibitory effect of CsA on NO production in C6 cells, suggesting calcineurin-independent mechanism of CsA action.
Details
- Title: Subtitle
- Cyclosporin A inhibits activation of inducible nitric oxide synthase in C6 glioma cell line
- Creators
- Vladimir Trajković - Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotića 1, 11000 Belgrade, YugoslaviaVladimir Badovinac - Institute for Biological Research, University of Belgrade, 29 Novembra 142, 11000 Belgrade, YugoslaviaVladimir Janković - Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotića 1, 11000 Belgrade, YugoslaviaMarija Mostarica Stojković - Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotića 1, 11000 Belgrade, Yugoslavia
- Resource Type
- Journal article
- Publication Details
- Brain research, Vol.816(1), pp.92-98
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0006-8993(98)01130-5
- PMID
- 9878697
- ISSN
- 0006-8993
- eISSN
- 1872-6240
- Language
- English
- Date published
- 1999
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047605102771
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