Journal article
Cytokine-induced phosphorylation of pp100 in FDC-ER cells is at tyrosine residues
Biochemical and biophysical research communications, Vol.188(3), pp.1040-1046
1992
DOI: 10.1016/0006-291X(92)91336-O
PMID: 1280128
Abstract
Using FDC-P1 cells stably transfected with a murine erythropoietin receptor cDNA as a model, we recently have shown that erythropoietin (EPO), IL-3 and GM-CSF each induce the rapid phosphorylation of a common cytosolic target, i.e., a Mr 100,000 phosphoprotein “pp100”. Presently, we demonstrate that cytokine-induced phosphorylation of pp100 is primarily at tyrosine residues. This is shown by Western blotting with the anti-phosphotyrosine antibody PY20, and by the resistance of [
32P]-pp100 to hydroxide-mediated hydrolysis of phosphates. These data, together with the recent observation by Linnekin et al. that
pp100
p97
apparently associates directly with EPO receptors, suggest that pp100 may comprise an immediate common component in the signal transduction pathways of EPO, IL-3, GM-CSF and possibly other type I/II cytokine receptors. Additional analyses suggest that pp100 is distinct from a previously described Mr 100,000 cytosolic target which is tyrosine phosphorylated in hematopoietic cells upon activation of T-cell receptors.
Details
- Title: Subtitle
- Cytokine-induced phosphorylation of pp100 in FDC-ER cells is at tyrosine residues
- Creators
- Fredrick W Quelle - Department of Molecular and Cell Biology, The Pennsylvania State University 408 South Frear Laboratory, University Park, PA 16802, USAMark Egerton - Child Health and Human Development, Cell Biology and Metabolism, National Institutes of Health, 900 Rockville Pike, Bethesda, MD 20892, USALawrence E Samelson - Child Health and Human Development, Cell Biology and Metabolism, National Institutes of Health, 900 Rockville Pike, Bethesda, MD 20892, USADon M Wojchowski - Department of Molecular and Cell Biology, The Pennsylvania State University 408 South Frear Laboratory, University Park, PA 16802, USA
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.188(3), pp.1040-1046
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0006-291X(92)91336-O
- PMID
- 1280128
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 1992
- Academic Unit
- Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040252202771
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