Cytomegalovirus and the role of interferon in the expression of tumor necrosis factor–related apoptosis-inducing ligand in the placenta

Janet I Andrews; Thomas S Griffith; Jeffery L Meier

doi:10.1016/j.ajog.2007.04.031

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Cytomegalovirus and the role of interferon in the expression of tumor necrosis factor–related apoptosis-inducing ligand in the placenta

Journal article

Peer reviewed

Cytomegalovirus and the role of interferon in the expression of tumor necrosis factor–related apoptosis-inducing ligand in the placenta

Janet I Andrews, Thomas S Griffith and Jeffery L Meier

American journal of obstetrics and gynecology, Vol.197(6), pp.608.e1-608.e6

2007

DOI: 10.1016/j.ajog.2007.04.031

PMID: 18060949

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Abstract

Cytomegalovirus infection causes adverse outcomes during pregnancy. Our objective was to determine the role of cytomegalovirus in modulating tumor necrosis factor (TNF)–related apoptosis-inducing ligand expression in the placenta. TNF-related apoptosis-inducing ligand messenger RNA and protein were quantified in cytomegalovirus-infected placental fibroblasts by polymerase chain reaction and enzyme-linked immunosorbent assays, respectively. Blocking antibodies against interferon and type I interferon receptor were applied to culture medium to characterize the role of type I interferon in cytomegalovirus-induced TNF-related apoptosis-inducing ligand upregulation. Expression of TNF-related apoptosis-inducing ligand messenger RNA and protein was increased in cytomegalovirus-infected placental fibroblasts, compared with uninfected controls. The cytomegalovirus-induced TNF-related apoptosis-inducing ligand messenger RNA upregulation was demonstrated across gestation, occurred in the absence of viral gene expression, and required cellular protein synthesis. TNF-related apoptosis-inducing ligand messenger RNA upregulation was markedly attenuated by inactivation of either type I interferon or its receptor. One mechanism by which cytomegalovirus infection causes unfavorable pregnancy outcomes may involve placental upregulation of TNF-related apoptosis-inducing ligand via an interferon-mediated pathway.

placenta

tumor necrosis factor-related apoptosis-inducing ligand

cytomegalovirus

interferon

Details

Title: Subtitle: Cytomegalovirus and the role of interferon in the expression of tumor necrosis factor–related apoptosis-inducing ligand in the placenta
Creators: Janet I Andrews - Department of Obstetrics and Gynecology, Carver College of Medicine, University of Iowa, Iowa City, IA
Thomas S Griffith - Department of Urology, Carver College of Medicine, University of Iowa, Iowa City, IA
Jeffery L Meier - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA
Resource Type: Journal article
Publication Details: American journal of obstetrics and gynecology, Vol.197(6), pp.608.e1-608.e6
DOI: 10.1016/j.ajog.2007.04.031
PMID: 18060949
NLM abbreviation: Am J Obstet Gynecol
ISSN: 0002-9378
eISSN: 1097-6868
Publisher: Mosby, Inc
Language: English
Date published: 2007
Academic Unit: Infectious Diseases; Epidemiology; Obstetrics and Gynecology; Internal Medicine
Record Identifier: 9984094378402771

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