Cytoskeletal and membrane bound antigens in human oral normal and malignant keratinocytes: an intensity of staining analysis

Xian-Jin Xie; M Fonzi; A Gasparoni; L Fonzi

Back

Journal article

Cytoskeletal and membrane bound antigens in human oral normal and malignant keratinocytes: an intensity of staining analysis

Xian-Jin Xie, M Fonzi, A Gasparoni and L Fonzi

Bulletin du Groupement international pour la recherche scientifique en stomatologie & odontologie, Vol.44(3), pp.88-96

10/2002

PMID: 12784584

View Online

Abstract

In this study, we tested the hypothesis that a significant difference exists in the integrated optical density (IOD) of membrane and cytoplasmic antigens in monolayer keratinocytes cultures. Oral normal and two malignant (SCC15 and SCC25) keratinocyte cultures were stained with antibodies specific for E-cadherin, beta-catenin, beta-1 integrin, cytokeratin (CK) 14, CK19, CK10/11 and involucrin. The IOD recorded (n = 12) was analyzed for significant differences using a two-way analysis of variance (significance level set at alpha = 0.05); antibodies and cell cultures were grouped according to Tukey's Group Comparison post-test. The majority of normal cell cultures exhibited E-cadherin, beta-catenin, involucrin and beta-1 integrin IOD values significantly higher than the two SCC cell lines. No definite staining pattern distinguished normal and malignant cells in relation to cytokeratins 14, 19 and 10/11. Our observations suggest that IOD measures constitute a good predictor of antigen steady state levels in monolayer cell cultures. According to these observations, SCC cell lines and normal cells appear to differ in the expression of E-cadherin, beta-catenin, beta-1 integrin and involucrin, although some variability within normal cells can also be observed.

Immunohistochemistry

Antibodies

Gingiva - immunology

Protein Precursors - analysis

Cytoskeleton - immunology

Coloring Agents

Humans

Trans-Activators - analysis

Gingiva - ultrastructure

Keratins - analysis

Antigens - analysis

Matched-Pair Analysis

Cell Culture Techniques

Tumor Cells, Cultured

Keratinocytes - ultrastructure

Cytoskeletal Proteins - analysis

beta Catenin

Cadherins - analysis

Integrin beta1 - analysis

Carcinoma, Squamous Cell - immunology

Keratinocytes - immunology

Microscopy, Confocal

Image Processing, Computer-Assisted

Carcinoma, Squamous Cell - ultrastructure

Cell Membrane - immunology

Antigens, Surface - analysis

Details

Title: Subtitle: Cytoskeletal and membrane bound antigens in human oral normal and malignant keratinocytes: an intensity of staining analysis
Creators: Xian-Jin Xie - Dows Institute for Dental Research, Iowa City, Iowa, USA
M Fonzi
A Gasparoni
L Fonzi
Resource Type: Journal article
Publication Details: Bulletin du Groupement international pour la recherche scientifique en stomatologie & odontologie, Vol.44(3), pp.88-96
Publisher: Belgium
PMID: 12784584
ISSN: 0250-4693
Language: English
Date published: 10/2002
Academic Unit: Preventive and Community Dentistry; Biostatistics; Dental Research
Record Identifier: 9983917679102771

Metrics

30 Record Views