Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells

Peter R Strege; Adrian N Holm; Adam Rich; Steven M Miller; Yijun Ou; Michael G Sarr; Gianrico Farrugia

doi:10.1152/ajpcell.00532.2001

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Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells

Journal article

Peer reviewed

Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells

Peter R Strege, Adrian N Holm, Adam Rich, Steven M Miller, Yijun Ou, Michael G Sarr and Gianrico Farrugia

American Journal of Physiology: Cell Physiology, Vol.284(1), pp.C60-66

01/2003

DOI: 10.1152/ajpcell.00532.2001

PMID: 12475760

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Abstract

A Na(+) current is present in human jejunal circular smooth muscle cells. The aim of the present study was to determine the role of the cytoskeleton in the regulation of the Na(+) current. Whole cell currents were recorded by using standard patch-clamp techniques with Cs(+) in the pipette to block K(+) currents. Cytochalasin D and gelsolin were used to disrupt the actin cytoskeleton and phalloidin to stabilize it. Colchicine was used to disassemble the microtubule cytoskeleton (and intermediate filaments) and paclitaxel to stabilize it. Acrylamide was used to disrupt the intermediate filament cytoskeleton. Perfusion of the recording chamber at 10 ml/min increased peak Na(+) current recorded from jejunal smooth muscle cells by 27 +/- 3%. Cytochalasin D and gelsolin abolished the perfusion-induced increase in Na(+) current, whereas incubation with phalloidin, colchicine, paclitaxel, or acrylamide had no effect. In conclusion, the Na(+) current expressed in human jejunal circular smooth muscle cells appears to be regulated by the cytoskeleton. An intact actin cytoskeleton is required for perfusion-induced activation of the Na(+) current.

Jejunum - physiology

Humans

Sodium Channels - physiology

Cytochalasin D - pharmacology

Action Potentials - physiology

Jejunum - drug effects

Muscle, Smooth - drug effects

Gelsolin - pharmacology

Patch-Clamp Techniques

Perfusion - adverse effects

Muscle, Smooth - physiology

Cytoskeleton - physiology

Jejunum - cytology

Muscle, Smooth - cytology

In Vitro Techniques

Action Potentials - drug effects

Cytoskeleton - drug effects

Details

Title: Subtitle: Cytoskeletal modulation of sodium current in human jejunal circular smooth muscle cells
Creators: Peter R Strege - Department of Physiology and Biophysics, Mayo Clinic and Foundation, 200 First Street SW, Rochester, MN 55905, USA
Adrian N Holm
Adam Rich
Steven M Miller
Yijun Ou
Michael G Sarr
Gianrico Farrugia
Resource Type: Journal article
Publication Details: American Journal of Physiology: Cell Physiology, Vol.284(1), pp.C60-66
DOI: 10.1152/ajpcell.00532.2001
PMID: 12475760
ISSN: 0363-6143
eISSN: 1522-1563
Grant note: DK 17238 / NIDDK NIH HHS DK 39337 / NIDDK NIH HHS DK 52766 / NIDDK NIH HHS DK 57061 / NIDDK NIH HHS
Language: English
Date published: 01/2003
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094379502771

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