Journal article
Cytotoxic T cell-resistant variants are selected in a virus-induced demyelinating disease
Immunity, Vol.5(3), pp.253-262
09/1996
DOI: 10.1016/S1074-7613(00)80320-9
PMID: 8808680
Abstract
C57BI/6 mice infected with mouse hepatitis virus, strain JHM (MHV-JHM) develop a chronic demyelinating encephalomyelitis. Infectious virus can be isolated only from symptomatic mice. In C57BI/6 mice, two CD8+ T cell epitopes within the MHV-JHM surface glycoprotein were previously identified. Here, we show that mutations in the RNA encoding the immunodominant of the epitopes are present in nearly all virus samples isolated from these mice. Mutations are not present in sequences flanking this epitope or in other CD8+ or CD4+ T cell epitopes. Furthermore, analysis of five peptides corresponding to variant epitopes in direct ex vivo cytotoxicity assays showed that each mutation caused a loss of epitope recognition. These results suggest that escape from CD8+ T cell recognition is necessary for enhanced virus replication and development of clinical disease in these MHV-JHM-infected mice.
Details
- Title: Subtitle
- Cytotoxic T cell-resistant variants are selected in a virus-induced demyelinating disease
- Creators
- L Pewe - Department of Pediatrics, University of Iowa, Iowa City 52242, USAG F WuE M BarnettR F CastroS Perlman
- Resource Type
- Journal article
- Publication Details
- Immunity, Vol.5(3), pp.253-262
- DOI
- 10.1016/S1074-7613(00)80320-9
- PMID
- 8808680
- NLM abbreviation
- Immunity
- ISSN
- 1074-7613
- eISSN
- 1097-4180
- Publisher
- United States
- Grant note
- NS 24401 / NINDS NIH HHS
- Language
- English
- Date published
- 09/1996
- Academic Unit
- Microbiology and Immunology; University of Iowa; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777348402771
Metrics
23 Record Views