Cytotoxicity, Differentiation, and Biocompatibility of Root-End Filling: A Comprehensive Study

Ignacio Jimenez-Bueno; Rene Garcia-Contreras; Benjamin Aranda-Herrera; Hiroshi Sakagami; Christian Andrea Lopez-Ayuso; Hiroshi Nakajima; Carlos A. Jurado; Hamid Nurrohman

doi:10.3390/biomimetics8070514

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Cytotoxicity, Differentiation, and Biocompatibility of Root-End Filling: A Comprehensive Study

Journal article

Open access

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Cytotoxicity, Differentiation, and Biocompatibility of Root-End Filling: A Comprehensive Study

Ignacio Jimenez-Bueno, Rene Garcia-Contreras, Benjamin Aranda-Herrera, Hiroshi Sakagami, Christian Andrea Lopez-Ayuso, Hiroshi Nakajima, Carlos A. Jurado and Hamid Nurrohman

Biomimetics (Basel, Switzerland), Vol.8(7), 514

10/29/2023

DOI: 10.3390/biomimetics8070514

PMCID: PMC10669418

PMID: 37999155

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Abstract

Assessing the biocompatibility of endodontic root-end filling materials through cell line responses is both essential and of utmost importance. This study aimed to the cytotoxicity of the type of cell death through apoptosis and autophagy, and odontoblast cell-like differentiation effects of MTA, zinc oxide–eugenol, and two experimental Portland cements modified with bismuth (Portland Bi) and barium (Portland Ba) on primary cell cultures. Material and methods: The cells corresponded to human periodontal ligament and gingival fibroblasts (HPLF, HGF), human pulp cells (HPC), and human squamous carcinoma cells from three different patients (HSC-2, -3, -4). The cements were inoculcated in different concentrations for cytotoxicity evaluation, DNA fragmentation in electrophoresis, apoptosis caspase activation, and autophagy antigen reaction, odontoblast-like cells were differentiated and tested for mineral deposition. The data were subject to a non-parametric test. Results: All cements caused a dose-dependent reduction in cell viability. Contact with zinc oxide–eugenol induced neither DNA fragmentation nor apoptotic caspase-3 activation and autophagy inhibitors (3-methyladenine, bafilomycin). Portland Bi accelerated significantly (p < 0.05) the differentiation of odontoblast-like cells. Within the limitation of this study, it was concluded that Portland cement with bismuth exhibits cytocompatibility and promotes odontoblast-like cell differentiation. This research contributes valuable insights into biocompatibility, suggesting its potential use in endodontic repair and biomimetic remineralization.

Details

Title: Subtitle: Cytotoxicity, Differentiation, and Biocompatibility of Root-End Filling: A Comprehensive Study
Creators: Ignacio Jimenez-Bueno - Universidad Autónoma del Estado de México
Rene Garcia-Contreras - Universidad Nacional Autónoma de México
Benjamin Aranda-Herrera - Universidad Nacional Autónoma de México
Hiroshi Sakagami - Meikai University
Christian Andrea Lopez-Ayuso - Universidad Nacional Autónoma de México
Hiroshi Nakajima - Meikai University
Carlos A. Jurado - University of Iowa
Hamid Nurrohman
Resource Type: Journal article
Publication Details: Biomimetics (Basel, Switzerland), Vol.8(7), 514
DOI: 10.3390/biomimetics8070514
PMID: 37999155
PMCID: PMC10669418
NLM abbreviation: Biomimetics (Basel)
ISSN: 2313-7673
eISSN: 2313-7673
Grant note: name: UNAM-DGAPA-PAPIME and PAPIIT, award: PE203622, IT200922
Language: English
Date published: 10/29/2023
Academic Unit: Prosthodontics
Record Identifier: 9984511952002771

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