Journal article
Cytotoxicity, mutagenicity, oxidative stress and mitochondrial impairment in human hepatoma (HepG2) cells exposed to copper oxide, copper-iron oxide and carbon nanoparticles
Ecotoxicology and environmental safety, Vol.189, pp.109982-109982
02/2020
DOI: 10.1016/j.ecoenv.2019.109982
PMID: 31830603
Abstract
The increasing application of nanomaterials in various fields such as drug delivery, cosmetics, disease detection, cancer treatment, food preservation etc. has resulted in high levels of engineered nanoparticles in the environment, thus leading to higher possibility of direct or indirect interactions between these particles and biological systems. In this study, the toxic effects of three commercially available nanomaterials; copper oxide nanoparticles, copper-iron oxide nanopowders and carbon nanopowders were determined in the human hepatoma HepG2 cells using various toxicological assays which are indicative of cytotoxicity (MTT and neutral red assays), mutagenicity (cytokinesis-block micronucleus assay), oxidative stress (total reactive oxygen species and superoxide anion production) and mitochondrial impairment (cellular oxygen consumption). There was increased cytotoxicity, mutagenicity, and mitochondrial impairment in the cells treated with higher concentrations of the nanomaterials, especially the copper oxide nanoparticles. The fold production of reactive oxygen species was similar at the concentrations tested in this study but longer exposure duration resulted in production of more superoxide anions. The results of this study showed that copper oxide nanoparticles are highly toxic to the human HepG2 cells, thus implying that the liver is a target organ in human for copper oxide nanoparticles toxicity.
•Wide applications of nanomaterials have led to their high levels in the environment.•Toxicological assays are required to determine their toxic effects.•CuO NPs, CuFe2O3 NPs and C-NPs were screened for their toxicities against HepG2 cells.•CuO NPs are highly toxic to human HepG2 cells.
Details
- Title: Subtitle
- Cytotoxicity, mutagenicity, oxidative stress and mitochondrial impairment in human hepatoma (HepG2) cells exposed to copper oxide, copper-iron oxide and carbon nanoparticles
- Creators
- Joseph A Adeyemi - Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/nº, CEP, 14040-903, Ribeirão Preto, São Paulo, BrazilAna Rita Thomazela Machado - Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/nº, CEP, 14040-903, Ribeirão Preto, São Paulo, BrazilAbayomi T Ogunjimi - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, 115 S Grand Avenue, Iowa City, IA, USALuciane Carla Alberici - Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/nº, CEP, 14040-903, Ribeirão Preto, São Paulo, BrazilLusania Maria Greggi Antunes - Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/nº, CEP, 14040-903, Ribeirão Preto, São Paulo, BrazilFernando Barbosa - Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café s/nº, CEP, 14040-903, Ribeirão Preto, São Paulo, Brazil
- Resource Type
- Journal article
- Publication Details
- Ecotoxicology and environmental safety, Vol.189, pp.109982-109982
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.ecoenv.2019.109982
- PMID
- 31830603
- ISSN
- 0147-6513
- eISSN
- 1090-2414
- Grant note
- DOI: 10.13039/501100001807, name: FAPESP, award: 2015/20725-5, 2018/24069-3; DOI: 10.13039/501100002322, name: CAPES, award: Code 001; DOI: 10.13039/501100003593, name: CNPq
- Language
- English
- Date published
- 02/2020
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984066111302771
Metrics
21 Record Views